| Literature DB >> 34941412 |
Pawel Roszak1, Jung-Ok Heo1,2, Bernhard Blob1, Koichi Toyokura1,3,4,5, Yuki Sugiyama1,6, Maria Angels de Luis Balaguer7, Winnie W Y Lau8, Fiona Hamey8, Jacopo Cirrone9, Ewelina Madej10, Alida M Bouatta11, Xin Wang2, Marjorie Guichard12,13, Robertas Ursache1, Hugo Tavares1,14, Kevin Verstaen15,16, Jos Wendrich17,18, Charles W Melnyk19, Yoshihisa Oda6,20, Dennis Shasha9, Sebastian E Ahnert1,21,22, Yvan Saeys15,16, Bert De Rybel17,18, Renze Heidstra23, Ben Scheres23,24, Guido Grossmann12,13, Ari Pekka Mähönen2, Philipp Denninger11, Berthold Göttgens8, Rosangela Sozzani7, Kenneth D Birnbaum25, Yrjö Helariutta1,2.
Abstract
In the plant meristem, tissue-wide maturation gradients are coordinated with specialized cell networks to establish various developmental phases required for indeterminate growth. Here, we used single-cell transcriptomics to reconstruct the protophloem developmental trajectory from the birth of cell progenitors to terminal differentiation in the Arabidopsis thaliana root. PHLOEM EARLY DNA-BINDING-WITH-ONE-FINGER (PEAR) transcription factors mediate lineage bifurcation by activating guanosine triphosphatase signaling and prime a transcriptional differentiation program. This program is initially repressed by a meristem-wide gradient of PLETHORA transcription factors. Only the dissipation of PLETHORA gradient permits activation of the differentiation program that involves mutual inhibition of early versus late meristem regulators. Thus, for phloem development, broad maturation gradients interface with cell-type-specific transcriptional regulators to stage cellular differentiation.Entities:
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Year: 2021 PMID： 34941412 PMCID： PMC8730638 DOI： 10.1126/science.aba5531
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728