An antiviral T-cell clone defines a functional supertypic specificity shared by different HLA-DR molecules from DR2-short, DRw11, and DRw13 haplotypes.

An influenza virus-specific HLA class II-restricted human T4+ clone (Ij) allows us to define a new functional supertypic HLA class II specificity shared by three different haplotypes. Influenza A virus-infected antigen-presenting cells of these three haplotypes, HLA-DR2 short, DRw11, and DRw13, are able to stimulate Ij cells. The same precise viral specificity is seen in all three cases. Proliferation inhibition experiments using HLA-specific monoclonal antibodies demonstrate that HLA-DR products are involved in all cases. However, according to the DR specificity of the antigen-presenting cell, differential blockings by a series of DR-specific monoclonal antibodies suggest that the functional epitope is shared by different HLA-DR molecules. This is confirmed by two-dimensional gel analysis of the HLA-DR beta chains expressed in the three haplotypes.