Yan Fu1,2, Xiang Gao3, Guang-Hui He4,2,5, Song Chen3,4,2, Zhao-Hui Gu1, Yue-Ling Zhang1, Li-Ying Li1. 1. Department of Ophthalmology, Baoding No.1 Central Hospital, Baoding 071000, Hebei Province, China. 2. Clinical College of Ophthalmology, Tianjin Medical University, Tianjin 300020, China. 3. College of Medicine, Nankai University, Tianjin 300071, China. 4. Tianjin Eye Hospital, Tianjin 300020, China. 5. Ophthalmic Center of Xinjiang Production and Construction Corps Hospital, Urumqi 830002, Xinjiang Uygur Autonomous Region, China.
Abstract
AIM: To assess the protective effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exs) in a diabetic rat model by using a variety of retinal bioassays. METHODS: hucMSCs were subjected to differential ultracentrifugation for the collection of exosomes, and transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) using a NanoSight analysis system and Western blotting (WB) were used to analyze the expression of surface marker proteins such as CD63, CD9 and Calnexin. Streptozotocin (STZ) was injected into the intraperitoneal cavity to establish a diabetic model. Rats were divided into a normal group, diabetic group and hucMSC-Ex group. Fundus fluorescein angiography (FFA), optical coherence tomography (OCT) and other live imaging methods were used to observe the fundus of the rats. Finally, the eyeballs of rats from each group were collected for hematoxylin-eosin (HE) staining to further analyze the retinal structure. RESULTS: Through TEM, NTA and WB, we successfully isolated hucMSC-Exs. Subsequent FFA and OCT confirmed that hucMSC-Exs effectively prevented early retinal vascular damage and thickening of the retina. Finally, HE staining of rat retinal sections revealed that exosomes effectively alleviated retinal structure disruption caused by diabetes. CONCLUSION: hucMSC-Exs have a protective effect on the retina in diabetic rat through FFA, OCT and HE staining. International Journal of Ophthalmology Press.
AIM: To assess the protective effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Exs) in a diabetic rat model by using a variety of retinal bioassays. METHODS: hucMSCs were subjected to differential ultracentrifugation for the collection of exosomes, and transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) using a NanoSight analysis system and Western blotting (WB) were used to analyze the expression of surface marker proteins such as CD63, CD9 and Calnexin. Streptozotocin (STZ) was injected into the intraperitoneal cavity to establish a diabetic model. Rats were divided into a normal group, diabetic group and hucMSC-Ex group. Fundus fluorescein angiography (FFA), optical coherence tomography (OCT) and other live imaging methods were used to observe the fundus of the rats. Finally, the eyeballs of rats from each group were collected for hematoxylin-eosin (HE) staining to further analyze the retinal structure. RESULTS: Through TEM, NTA and WB, we successfully isolated hucMSC-Exs. Subsequent FFA and OCT confirmed that hucMSC-Exs effectively prevented early retinal vascular damage and thickening of the retina. Finally, HE staining of rat retinal sections revealed that exosomes effectively alleviated retinal structure disruption caused by diabetes. CONCLUSION: hucMSC-Exs have a protective effect on the retina in diabetic rat through FFA, OCT and HE staining. International Journal of Ophthalmology Press.
Entities:
Keywords:
diabetic retinopathy; exosomes; fundus fluorescein angiography; human umbilical cord mesenchymal stem cells; optical coherence tomography; rat
Authors: Chieh Allen Lee; Guangyuan Li; Mansi D Patel; J Mark Petrash; Beth Ann Benetz; Alex Veenstra; Jaume Amengual; Johannes von Lintig; Christopher J Burant; Johnny Tang; Timothy S Kern Journal: Invest Ophthalmol Vis Sci Date: 2014-05-02 Impact factor: 4.799
Authors: Valentin M Sluch; Xitiz Chamling; Melissa M Liu; Cynthia A Berlinicke; Jie Cheng; Katherine L Mitchell; Derek S Welsbie; Donald J Zack Journal: Stem Cells Transl Med Date: 2017-10-10 Impact factor: 6.940