Literature DB >> 3490668

Identification of two anti-parallel beta-sheet conformations in the solution structure of murine epidermal growth factor by proton magnetic resonance.

G T Montelione, K Wüthrich, E C Nice, A W Burgess, H A Scheraga.   

Abstract

Epidermal growth factor (EGF) is a small mitogenic protein. Proteins with sequence homology with EGF or with its membrane-bound protein receptor have been proposed to play a role in oncogenesis. This report describes solution NMR data that provide evidence that the solution conformation of murine EGF includes an anti-parallel beta-sheet structure involving residues S2-P4, V19-I23, and S28-N32; a small anti-parallel beta-sheet involving residues Y37-S38 and T44-R45; and a multiple-bend (or short irregular helix) structure for residues C6-C14 that is disulfide bonded to the V19-I23/S28-N32 beta-sheet. Implications of these results for structure and function studies of EGF and for molecular design of EGF and homologous alpha-type transforming growth factors are discussed.

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Year:  1986        PMID： 3490668      PMCID： PMC386977          DOI： 10.1073/pnas.83.22.8594

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  31 in total

1.  Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal.

Authors:  S COHEN
Journal:  J Biol Chem       Date:  1962-05       Impact factor: 5.157

2.  Growth factors from murine sarcoma virus-transformed cells.

Authors:  J E de Larco; G J Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

3.  Epidermal growth factor. Location of disulfide bonds.

Authors:  C R Savage; J H Hash; S Cohen
Journal:  J Biol Chem       Date:  1973-11-25       Impact factor: 5.157

4.  Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor.

Authors:  G Wagner; K Wüthrich
Journal:  J Mol Biol       Date:  1982-03-05       Impact factor: 5.469

5.  Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.

Authors:  G J Todaro; C Fryling; J E De Larco
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Synthesis of a human insulin gene. V. Enzymatic assembly, cloning and characterization of the human proinsulin DNA.

Authors:  R Brousseau; R Scarpulla; W Sung; H M Hsiung; S A Narang; R Wu
Journal:  Gene       Date:  1982-03       Impact factor: 3.688

7.  Secondary structure in the solution conformation of the proteinase inhibitor IIA from bull seminal plasma by nuclear magnetic resonance.

Authors:  M P Williamson; D Marion; K Wüthrich
Journal:  J Mol Biol       Date:  1984-03-05       Impact factor: 5.469

8.  Two forms of murine epidermal growth factor: rapid separation by using reverse-phase HPLC.

Authors:  A W Burgess; J Knesel; L G Sparrow; N A Nicola; E C Nice
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

9.  Epidermal growth factor from the mouse. Physical evidence for a tiered beta-sheet domain: two-dimensional NMR correlated spectroscopy and nuclear Overhauser experiments on backbone amide protons.

Authors:  K H Mayo
Journal:  Biochemistry       Date:  1985-07-02       Impact factor: 3.162

10.  Human transforming growth factor-alpha causes precocious eyelid opening in newborn mice.

Authors:  J M Smith; M B Sporn; A B Roberts; R Derynck; M E Winkler; H Gregory
Journal:  Nature       Date:  1985 Jun 6-12       Impact factor: 49.962
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  14 in total

1.  Structure-function analysis of human transforming growth factor-alpha by site-directed mutagenesis.

Authors:  J A Feild; R H Reid; D J Rieman; T P Kline; G Sathe; R G Greig; M A Anzano
Journal:  Biochem J       Date:  1992-04-01       Impact factor: 3.857

2.  Simulated annealing with restrained molecular dynamics using CONGEN: energy refinement of the NMR solution structures of epidermal and type-alpha transforming growth factors.

Authors:  R Tejero; D Bassolino-Klimas; R E Bruccoleri; G T Montelione
Journal:  Protein Sci       Date:  1996-04       Impact factor: 6.725

3.  Structure-function studies of mEGF: probing the type I beta-turn between residues 25 and 26.

Authors:  C C Lester; B Wang; R Wu; H A Scheraga
Journal:  J Protein Chem       Date:  1995-11

4.  Solution conformations of the B-loop fragments of human transforming growth factor alpha and epidermal growth factor by 1H nuclear magnetic resonance and restrained molecular dynamics.

Authors:  K H Han; J L Syi; B R Brooks; J A Ferretti
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

5.  Sequence-specific 1H-n.m.r. assignments and peptide backbone conformation in rat epidermal growth factor.

Authors:  K H Mayo; R C Cavalli; A R Peters; R Boelens; R Kaptein
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

6.  Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.

Authors:  E Lazar; E Vicenzi; E Van Obberghen-Schilling; B Wolff; S Dalton; S Watanabe; M B Sporn
Journal:  Mol Cell Biol       Date:  1989-02       Impact factor: 4.272

7.  Solution structure of murine epidermal growth factor: determination of the polypeptide backbone chain-fold by nuclear magnetic resonance and distance geometry.

Authors:  G T Montelione; K Wüthrich; E C Nice; A W Burgess; H A Scheraga
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

8.  Role of the 6-20 disulfide bridge in the structure and activity of epidermal growth factor.

Authors:  K J Barnham; A M Torres; D Alewood; P F Alewood; T Domagala; E C Nice; R S Norton
Journal:  Protein Sci       Date:  1998-08       Impact factor: 6.725

9.  Combined use of 13C chemical shift and 1H alpha-13C alpha heteronuclear NOE data in monitoring a protein NMR structure refinement.

Authors:  B Celda; C Biamonti; M J Arnau; R Tejero; G T Montelione
Journal:  J Biomol NMR       Date:  1995-02       Impact factor: 2.835

10.  Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.

Authors:  E Lazar; S Watanabe; S Dalton; M B Sporn
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272
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