Literature DB >> 34905164

Data-Independent Acquisition Mass Spectrometry-Based Deep Proteome Analysis for Hydrophobic Proteins from Dried Blood Spots Enriched by Sodium Carbonate Precipitation.

Daisuke Nakajima1, Osamu Ohara1, Yusuke Kawashima2.   

Abstract

Dried blood spots (DBS) are widely used for screening molecular profiles, including enzymatic activity. However, hydrophilic proteins present in large amounts in blood inhibit detection of other proteins in DBS by liquid chromatography-mass spectrometry (LC-MS/MS) without preenrichment. Sodium carbonate precipitation (SCP) can concentrate hydrophobic proteins from DBS and effectively remove soluble hydrophilic proteins. Furthermore, SCP combination with data-independent acquisition (DIA) for quantitative LC-MS/MS enhanced the proteome analysis sensitivity and quantification limits. In this protocol, we have described in detail a simple preenrichment method using SCP and a deep proteome analysis method for LC-MS/MS data using DIA.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Clinical proteomics; Data-independent acquisition; Dried blood spots; Newborn screening; Sodium carbonate

Mesh：

Substances：

Year:  2022        PMID： 34905164     DOI： 10.1007/978-1-0716-1936-0_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  21 in total

Review 1.  The analysis of dried blood spot samples using liquid chromatography tandem mass spectrometry.

Authors:  Brian G Keevil
Journal:  Clin Biochem       Date:  2010-07-01       Impact factor: 3.281

Review 2.  What a drop can do: dried blood spots as a minimally invasive method for integrating biomarkers into population-based research.

Authors:  Thomas W McDade; Sharon Williams; J Josh Snodgrass
Journal:  Demography       Date:  2007-11

3.  Enhanced detection of low abundance human plasma proteins using a tandem IgY12-SuperMix immunoaffinity separation strategy.

Authors:  Wei-Jun Qian; David T Kaleta; Brianne O Petritis; Hongliang Jiang; Tao Liu; Xu Zhang; Heather M Mottaz; Susan M Varnum; David G Camp; Lei Huang; Xiangming Fang; Wei-Wei Zhang; Richard D Smith
Journal:  Mol Cell Proteomics       Date:  2008-07-15       Impact factor: 5.911

Review 4.  Dried blood spot sampling in combination with LC-MS/MS for quantitative analysis of small molecules.

Authors:  Wenkui Li; Francis L S Tse
Journal:  Biomed Chromatogr       Date:  2010-01       Impact factor: 1.902

Review 5.  Direct analysis of dried blood spots coupled with mass spectrometry: concepts and biomedical applications.

Authors:  Julien Déglon; Aurélien Thomas; Patrice Mangin; Christian Staub
Journal:  Anal Bioanal Chem       Date:  2011-06-25       Impact factor: 4.142

Review 6.  The role of protein and peptide separation before mass spectrometry analysis in clinical proteomics.

Authors:  Serena Camerini; Pierluigi Mauri
Journal:  J Chromatogr A       Date:  2014-12-18       Impact factor: 4.759

7.  High Field Asymmetric Waveform Ion Mobility Spectrometry in Nontargeted Bottom-up Proteomics of Dried Blood Spots.

Authors:  Cecilie Rosting; Jinglei Yu; Helen J Cooper
Journal:  J Proteome Res       Date:  2018-05-09       Impact factor: 4.466

8.  Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics.

Authors:  Daisuke Nakajima; Yusuke Kawashima; Hirofumi Shibata; Takahiro Yasumi; Masahiko Isa; Kazushi Izawa; Ryuta Nishikomori; Toshio Heike; Osamu Ohara
Journal:  J Proteome Res       Date:  2020-06-04       Impact factor: 4.466

9.  Comparison of strong cation exchange and SDS-PAGE fractionation for analysis of multiprotein complexes.

Authors:  Sudipto Das; Allen D Bosley; Xiaoying Ye; King C Chan; Isabel Chu; Jeffery E Green; Haleem J Issaq; Timothy D Veenstra; Thorkell Andresson
Journal:  J Proteome Res       Date:  2010-11-09       Impact factor: 4.466

10.  Dried blood spot proteomics: surface extraction of endogenous proteins coupled with automated sample preparation and mass spectrometry analysis.

Authors:  Nicholas J Martin; Josephine Bunch; Helen J Cooper
Journal:  J Am Soc Mass Spectrom       Date:  2013-06-01       Impact factor: 3.109
View more
  1 in total

Review 1.  COVIDomics: The Proteomic and Metabolomic Signatures of COVID-19.

Authors:  Michele Costanzo; Marianna Caterino; Roberta Fedele; Armando Cevenini; Mariarca Pontillo; Lucia Barra; Margherita Ruoppolo
Journal:  Int J Mol Sci       Date:  2022-02-22       Impact factor: 5.923
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.