Literature DB >> 3490427

Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits.

J M Saunders, J D Folds.   

Abstract

Sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblotting were used to characterise isolates of Haemophilus ducreyi. Isolates of H ducreyi were heterogeneous in protein composition, but isolates from single outbreaks appeared similar both in protein profiles and antigenic analysis. Rabbits immunised with H ducreyi responded with a vigorous humoral immune response in which multiple antigenic polypeptides were detected. The most prominent antigens had molecular masses of 67, 42, 22.5, and 20 kilodaltons.

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Year:  1986        PMID: 3490427      PMCID: PMC1011985          DOI: 10.1136/sti.62.5.321

Source DB:  PubMed          Journal:  Genitourin Med        ISSN: 0266-4348


  13 in total

1.  An indirect fluorescent antibody technique for Haemophilus ducreyi.

Authors:  G A Denys; T A Chapel; C D Jeffries
Journal:  Health Lab Sci       Date:  1978-07

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Chancroid and Haemophilus ducreyi.

Authors:  A R Ronald; F A Plummer
Journal:  Ann Intern Med       Date:  1985-05       Impact factor: 25.391

4.  Monoclonal antibodies reactive with all strains of Haemophilus ducreyi.

Authors:  E J Hansen; T A Loftus
Journal:  Infect Immun       Date:  1984-04       Impact factor: 3.441

5.  Humoral response of the mouse to Treponema pallidum.

Authors:  J M Saunders; J D Folds
Journal:  Genitourin Med       Date:  1985-08

6.  How reliable is the morphological diagnosis of penile ulcerations?

Authors:  T A Chapel; W J Brown; C Jeffres; J A Stewart
Journal:  Sex Transm Dis       Date:  1977 Oct-Dec       Impact factor: 2.830

7.  Pathogenic microbial flora of genital ulcers in Sheffield with particular reference to herpes simplex virus and Haemophilus ducreyi.

Authors:  G R Kinghorn; S Hafiz; M G McEntegart
Journal:  Br J Vener Dis       Date:  1982-12

8.  Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi.

Authors:  G W Hammond; C J Lian; J C Wilt; A R Ronald
Journal:  J Clin Microbiol       Date:  1978-01       Impact factor: 5.948

9.  Characterization of cell proteins of Haemophilus ducreyi by polyacrylamide gel electrophoresis.

Authors:  J A Odumeru; A R Ronald; W L Albritton
Journal:  J Infect Dis       Date:  1983-10       Impact factor: 5.226

10.  Isolation and cultivation of Haemophilus ducreyi.

Authors:  T R Oberhofer; A E Back
Journal:  J Clin Microbiol       Date:  1982-04       Impact factor: 5.948

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  11 in total

1.  The major outer membrane protein of Haemophilus ducreyi consists of two OmpA homologs.

Authors:  J Klesney-Tait; T J Hiltke; I Maciver; S M Spinola; J D Radolf; E J Hansen
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

2.  DNA probes for the identification of Haemophilus ducreyi.

Authors:  L M Parsons; M Shayegani; A L Waring; L H Bopp
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

3.  Lectin typing of Haemophilus ducreyi.

Authors:  H C Korting; D Abeck; A P Johnson; R C Ballard; D Taylor-Robinson; O Braun-Falco
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-10       Impact factor: 3.267

4.  Antigenic analysis of Haemophilus ducreyi by Western blotting.

Authors:  D Abeck; A P Johnson; D Taylor-Robinson
Journal:  Epidemiol Infect       Date:  1988-08       Impact factor: 2.451

5.  Molecular analysis of the Haemophilus ducreyi groE heat shock operon.

Authors:  L M Parsons; A L Waring; M Shayegani
Journal:  Infect Immun       Date:  1992-10       Impact factor: 3.441

6.  Characterization of an 18,000-molecular-weight outer membrane protein of Haemophilus ducreyi that contains a conserved surface-exposed epitope.

Authors:  S M Spinola; G E Griffiths; J Bogdan; M A Menegus
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

7.  The major outer membrane protein of Haemophilus ducreyi is a member of the OmpA family of proteins.

Authors:  S M Spinola; G E Griffiths; K L Shanks; M S Blake
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441

8.  Neutralizing antibodies to Haemophilus ducreyi cytotoxin.

Authors:  T Lagergård; M Purvén
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441

9.  Antigenic diversity in Haemophilus ducreyi as shown by western blot (immunoblot) analysis.

Authors:  E L Roggen; S De Breucker; E van Dyck; P Piot
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

Review 10.  Biology of Haemophilus ducreyi.

Authors:  W L Albritton
Journal:  Microbiol Rev       Date:  1989-12
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