Literature DB >> 3042435

Antigenic analysis of Haemophilus ducreyi by Western blotting.

D Abeck1, A P Johnson, D Taylor-Robinson.   

Abstract

Twenty-one strains of Haemophilus ducreyi were analysed by Western blotting using two antisera produced in mice. Common antigens of molecular weights 58, 46, 41, 28, 22 and 16 kDa were detected in all the strains. The antigens were protein in nature, since they could not be detected in whole-cell lysates which had been treated with proteinase K. The H. ducreyi strains showed antigenic cross-reactivity with strains of H. influenzae and H. parainfluenzae, but showed minimal or no cross-reactivity with seven other species of bacteria.

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Year:  1988        PMID: 3042435      PMCID: PMC2249325          DOI: 10.1017/s0950268800029319

Source DB:  PubMed          Journal:  Epidemiol Infect        ISSN: 0950-2688            Impact factor:   2.451


  8 in total

1.  Antimicrobial susceptibility of Haemophilus ducreyi.

Authors:  G W Hammond; C J Lian; J C Wilt; A R Ronald
Journal:  Antimicrob Agents Chemother       Date:  1978-04       Impact factor: 5.191

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

Review 3.  Microbial surfaces in relation to pathogenicity.

Authors:  H Smith
Journal:  Bacteriol Rev       Date:  1977-06

4.  Characterization of cell proteins of Haemophilus ducreyi by polyacrylamide gel electrophoresis.

Authors:  J A Odumeru; A R Ronald; W L Albritton
Journal:  J Infect Dis       Date:  1983-10       Impact factor: 5.226

5.  Antimicrobial susceptibility and characterization of outer membrane proteins of Haemophilus ducreyi isolated in Thailand.

Authors:  D N Taylor; P Echeverria; S Hanchalay; C Pitarangsi; L Slootmans; P Piot
Journal:  J Clin Microbiol       Date:  1985-03       Impact factor: 5.948

6.  Immunoblot analysis of antigens associated with Haemophilus ducreyi using serum from immunised rabbits.

Authors:  J M Saunders; J D Folds
Journal:  Genitourin Med       Date:  1986-10

7.  Detection of a species-specific antigen of Gardnerella vaginalis by Western blot analysis.

Authors:  Y L Boustouller; A P Johnson; D T Taylor-Robinson
Journal:  J Gen Microbiol       Date:  1986-07

8.  Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.

Authors:  P J Hitchcock; T M Brown
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

  8 in total
  5 in total

1.  The major outer membrane protein of Haemophilus ducreyi consists of two OmpA homologs.

Authors:  J Klesney-Tait; T J Hiltke; I Maciver; S M Spinola; J D Radolf; E J Hansen
Journal:  J Bacteriol       Date:  1997-03       Impact factor: 3.490

2.  Molecular analysis of the Haemophilus ducreyi groE heat shock operon.

Authors:  L M Parsons; A L Waring; M Shayegani
Journal:  Infect Immun       Date:  1992-10       Impact factor: 3.441

3.  Characterization of an 18,000-molecular-weight outer membrane protein of Haemophilus ducreyi that contains a conserved surface-exposed epitope.

Authors:  S M Spinola; G E Griffiths; J Bogdan; M A Menegus
Journal:  Infect Immun       Date:  1992-02       Impact factor: 3.441

4.  The major outer membrane protein of Haemophilus ducreyi is a member of the OmpA family of proteins.

Authors:  S M Spinola; G E Griffiths; K L Shanks; M S Blake
Journal:  Infect Immun       Date:  1993-04       Impact factor: 3.441

Review 5.  Biology of Haemophilus ducreyi.

Authors:  W L Albritton
Journal:  Microbiol Rev       Date:  1989-12
  5 in total

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