PURPOSE: Females and males respond differently to a number of systemic viral infections. Differences between females and males with respect to the severity of keratitis caused by Gram-negative bacteria such as Serratia marcescens are less well established. METHODS: In this study, we injected female and male New Zealand White rabbit corneas with a keratitis isolate of S. marcescens and evaluated the eyes after 48 hours for a number of clinical and microbiological parameters. RESULTS: No statistical differences in bacterial burden and corneal scores were recorded between female and male rabbits although there was a non-significant trend toward a higher frequency of female rabbits demonstrating hypopyons. CONCLUSIONS: This data suggests that for experimental bacterial keratitis studies involving Gram-negative rods, a single sex or mixed group of rabbit is sufficient for evaluating pathology and bacterial burdens. This will reduce the number of animals used for subsequent studies.
PURPOSE: Females and males respond differently to a number of systemic viral infections. Differences between females and males with respect to the severity of keratitis caused by Gram-negative bacteria such as Serratia marcescens are less well established. METHODS: In this study, we injected female and male New Zealand White rabbit corneas with a keratitis isolate of S. marcescens and evaluated the eyes after 48 hours for a number of clinical and microbiological parameters. RESULTS: No statistical differences in bacterial burden and corneal scores were recorded between female and male rabbits although there was a non-significant trend toward a higher frequency of female rabbits demonstrating hypopyons. CONCLUSIONS: This data suggests that for experimental bacterial keratitis studies involving Gram-negative rods, a single sex or mixed group of rabbit is sufficient for evaluating pathology and bacterial burdens. This will reduce the number of animals used for subsequent studies.
Authors: Sharon Altmann; Andrew Emanuel; Megan Toomey; Kim McIntyre; Jill Covert; Richard Redd Dubielzig; Gary Leatherberry; Christopher J Murphy; Shantha Kodihalli; Curtis R Brandt Journal: Invest Ophthalmol Vis Sci Date: 2010-04-07 Impact factor: 4.799
Authors: Eric G Romanowski; Nicholas A Stella; John E Romanowski; Kathleen A Yates; Deepinder K Dhaliwal; Anthony J St Leger; Robert M Q Shanks Journal: Infect Immun Date: 2021-07-15 Impact factor: 3.441