Literature DB >> 34845692

Detection of Viroids by RT-PCR.

Nerida J Donovan1, Grant A Chambers2, Mengji Cao3.   

Abstract

Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Gel electrophoresis; Primers; Reverse transcription-polymerase chain reaction (RT-PCR); Viroid detection

Mesh：

Substances：

Year:  2022        PMID： 34845692     DOI： 10.1007/978-1-0716-1464-8_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Dahlia latent viroid: a recombinant new species of the family Pospiviroidae posing intriguing questions about its origin and classification.

Authors:  Jacobus Th J Verhoeven; Ellis T M Meekes; Johanna W Roenhorst; Ricardo Flores; Pedro Serra
Journal:  J Gen Virol       Date:  2012-12-19       Impact factor: 3.891

2.  Identification and characterization of a new coleviroid (CbVd-5).

Authors:  Wan-Ying Hou; Teruo Sano; Feng Li; Zu-Jian Wu; Li Li; Shi-Fang Li
Journal:  Arch Virol       Date:  2008-12-27       Impact factor: 2.574

3.  Deep sequencing of viroid-derived small RNAs from grapevine provides new insights on the role of RNA silencing in plant-viroid interaction.

Authors:  Beatriz Navarro; Vitantonio Pantaleo; Andreas Gisel; Simon Moxon; Tamas Dalmay; György Bisztray; Francesco Di Serio; József Burgyán
Journal:  PLoS One       Date:  2009-11-05       Impact factor: 3.240
  3 in total

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