Mara Luana Batista Severo1, Stéfanie Thieme2, Felipe Martins Silveira2,3, Raquel Padilha Martins Tavares4, Amanda Katarinny Goes Gonzaga5, Silvana Maria Zucolotto4, Aurigena Antunes de Araújo1, Marco Antonio Trevizani Martins2, Manoela Domingues Martins2,3, Éricka Janine Dantas da Silveira6. 1. Dentistry Department, Postgraduate Program in Dental Sciences, Federal University of Rio Grande Norte, Av. Senador Salgado Filho, Lagoa Nova, Natal, RN, 1787, Brazil. 2. Department of Oral Pathology, Dental School, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil. 3. Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil. 4. Department of Pharmacy, School of Pharmacy, Federal University of Rio Grande Norte, Natal, Brazil. 5. Department of Dentistry, UNIFACISA University Center, Campina Grande, PB, Brazil. 6. Dentistry Department, Postgraduate Program in Dental Sciences, Federal University of Rio Grande Norte, Av. Senador Salgado Filho, Lagoa Nova, Natal, RN, 1787, Brazil. ericka_janine@yahoo.com.br.
Abstract
PURPOSE: This study aimed to evaluate the effects and mechanisms of action of royal jelly (RJ) and propolis compared to photobiomodulation therapy (PBMT) in an animal model of 5-fluorouracil-related oral mucositis (OM). METHODS: Seventy-two male Wistar rats were randomly allocated to four groups (n = 18 each): control (no treatment), PBMT (intraoral laser, 6 J/cm2), RJ, and propolis. On days 0 and 2, the animals received an injection of 5-fluorouracil (5-FU). The buccal mucosa was scratched (days 3 and 4) and the treatments were initiated on day 5. Six animals of each group were euthanized on days 8, 10, and 14. Phytochemical analysis (thin-layer chromatography, TLC) and clinical, histopathological, and immunohistochemical analysis of pS6, pAKT, and NF-κB were performed, and oxidative stress markers were also investigated. RESULTS: TLC revealed the presence of large amounts of sucrose (Rf 0.34) in RJ and of flavonoids in propolis. Lower clinical OM scores were observed on day 8, and improved morphological data were observed on day 10 in the PBMT, RJ, and propolis groups (p < 0.05). On day 8, immunoexpression of pS6, pAKT, and NF-κB was increased compared to control. On day 14, reduced glutathione (GSH) antioxidant levels were increased in the propolis group compared to control (p < 0.05). CONCLUSIONS: Our results showed that RJ and propolis, as well as PBMT, are effective in the treatment of OM. Considering that some patients who develop OM do not have access to PBMT, the present study demonstrated that topical application of RJ and propolis may be an important alternative for the treatment of OM.
PURPOSE: This study aimed to evaluate the effects and mechanisms of action of royal jelly (RJ) and propolis compared to photobiomodulation therapy (PBMT) in an animal model of 5-fluorouracil-related oral mucositis (OM). METHODS: Seventy-two male Wistar rats were randomly allocated to four groups (n = 18 each): control (no treatment), PBMT (intraoral laser, 6 J/cm2), RJ, and propolis. On days 0 and 2, the animals received an injection of 5-fluorouracil (5-FU). The buccal mucosa was scratched (days 3 and 4) and the treatments were initiated on day 5. Six animals of each group were euthanized on days 8, 10, and 14. Phytochemical analysis (thin-layer chromatography, TLC) and clinical, histopathological, and immunohistochemical analysis of pS6, pAKT, and NF-κB were performed, and oxidative stress markers were also investigated. RESULTS: TLC revealed the presence of large amounts of sucrose (Rf 0.34) in RJ and of flavonoids in propolis. Lower clinical OM scores were observed on day 8, and improved morphological data were observed on day 10 in the PBMT, RJ, and propolis groups (p < 0.05). On day 8, immunoexpression of pS6, pAKT, and NF-κB was increased compared to control. On day 14, reduced glutathione (GSH) antioxidant levels were increased in the propolis group compared to control (p < 0.05). CONCLUSIONS: Our results showed that RJ and propolis, as well as PBMT, are effective in the treatment of OM. Considering that some patients who develop OM do not have access to PBMT, the present study demonstrated that topical application of RJ and propolis may be an important alternative for the treatment of OM.
Authors: Stéfanie Thieme; Julia Turra Ribeiro; Bernardo Gindri Dos Santos; Renata de Almeida Zieger; Mara Luana Batista Severo; Marco Antonio Trevizani Martins; Cristiane Matté; Manoela Domingues Martins Journal: Support Care Cancer Date: 2019-06-04 Impact factor: 3.603
Authors: Wanessa Miranda-Silva; Wagner Gomes-Silva; Yehuda Zadik; Noam Yarom; Abdul Rahman Al-Azri; Catherine H L Hong; Anura Ariyawardana; Deborah P Saunders; M Elvira Correa; Praveen R Arany; Joanne Bowen; Karis Kin Fong Cheng; Wim J E Tissing; Paolo Bossi; Sharon Elad Journal: Support Care Cancer Date: 2020-11-06 Impact factor: 3.603