Stéfanie Thieme1, Julia Turra Ribeiro1, Bernardo Gindri Dos Santos2, Renata de Almeida Zieger1, Mara Luana Batista Severo1, Marco Antonio Trevizani Martins1,3, Cristiane Matté2,4, Manoela Domingues Martins5,6,7,8. 1. Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 2. Postgraduate Program in Biological Sciences: Biochemistry, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 3. Department of Oral Medicine, Porto Alegre Clinics Hospital (HCPA/UFRGS), Porto Alegre, RS, Brazil. 4. Postgraduate Program in Biological Sciences: Physiology, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. 5. Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. manomartins@gmail.com. 6. Department of Oral Medicine, Porto Alegre Clinics Hospital (HCPA/UFRGS), Porto Alegre, RS, Brazil. manomartins@gmail.com. 7. Experimental Pathology Unit, Porto Alegre Clinics Hospital, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil. manomartins@gmail.com. 8. Faculdade de Odontologia, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2492, sala, 503, CEP: 90035-003, Santana, Porto Alegre, RS, Brazil. manomartins@gmail.com.
Abstract
PURPOSE: The aim of the present study was to compare the effect of intraoral (IO) and extraoral (EO) diode laser irradiation on oral mucositis (OM) induced by 5-fluorouracil (5-FU) in rats. METHODS: Animals (n = 78) were divided into the following groups: negative control (NC), positive control (PC), IO 6 J/cm2, EO with 6 J/cm2 (EO 6 J/cm2), and 12 J/cm2 (EO 12 J/cm2). OM was induced with an intraperitoneal injection of 5-FU and scarification of the buccal mucosa. Over the following 14 days, animals received photobiomodulation (PBM) daily. Clinical and histological evaluation was done by scores at days 8, 10, and 14. The redox state was evaluated by reactive species levels, antioxidant network, and immunohistochemistry analysis. RESULTS: Clinically, on day 8, PBM groups showed lower scores of OM with EO 6 J/cm2 presenting a significantly lower degree compared to PC (p < 0.05). On days 10 and 14, all PBM groups exhibited improvement of OM compared to PC (p < 0.01). On day 8, all PBM groups exhibited an accelerated healing process compared to PC (p < 0.01) and reduction of reactive species (p < 0.001). Also, all PBM groups demonstrated higher levels of antioxidant GPx compared to PC (p < 0.001). Analysis of nitrotyrosine revealed that on day 14, this protein damage marker was significantly reduced in the EO 6 J/cm2 group (p > 0.05). CONCLUSIONS: An EO diode laser protocol promoted positive effects in the clinical, histopathological, and redox state in OM induced by 5-FU in rats. Among the EO protocols, EO 6 J/cm2 showed the most encouraging results.
PURPOSE: The aim of the present study was to compare the effect of intraoral (IO) and extraoral (EO) diode laser irradiation on oral mucositis (OM) induced by 5-fluorouracil (5-FU) in rats. METHODS: Animals (n = 78) were divided into the following groups: negative control (NC), positive control (PC), IO 6 J/cm2, EO with 6 J/cm2 (EO 6 J/cm2), and 12 J/cm2 (EO 12 J/cm2). OM was induced with an intraperitoneal injection of 5-FU and scarification of the buccal mucosa. Over the following 14 days, animals received photobiomodulation (PBM) daily. Clinical and histological evaluation was done by scores at days 8, 10, and 14. The redox state was evaluated by reactive species levels, antioxidant network, and immunohistochemistry analysis. RESULTS: Clinically, on day 8, PBM groups showed lower scores of OM with EO 6 J/cm2 presenting a significantly lower degree compared to PC (p < 0.05). On days 10 and 14, all PBM groups exhibited improvement of OM compared to PC (p < 0.01). On day 8, all PBM groups exhibited an accelerated healing process compared to PC (p < 0.01) and reduction of reactive species (p < 0.001). Also, all PBM groups demonstrated higher levels of antioxidant GPx compared to PC (p < 0.001). Analysis of nitrotyrosine revealed that on day 14, this protein damage marker was significantly reduced in the EO 6 J/cm2 group (p > 0.05). CONCLUSIONS: An EO diode laser protocol promoted positive effects in the clinical, histopathological, and redox state in OM induced by 5-FU in rats. Among the EO protocols, EO 6 J/cm2 showed the most encouraging results.
Authors: Sharon Elad; Praveen Arany; Rene-Jean Bensadoun; Joel B Epstein; Andrei Barasch; Judith Raber-Durlacher Journal: Support Care Cancer Date: 2018-05-22 Impact factor: 3.603
Authors: V Lima; G A C Brito; F Q Cunha; C G Rebouças; B A A Falcão; R F Augusto; M L P Souza; B T Leitão; R A Ribeiro Journal: Eur J Oral Sci Date: 2005-06 Impact factor: 2.612
Authors: Judith A E M Zecha; Judith E Raber-Durlacher; Raj G Nair; Joel B Epstein; Stephen T Sonis; Sharon Elad; Michael R Hamblin; Andrei Barasch; Cesar A Migliorati; Dan M J Milstein; Marie-Thérèse Genot; Liset Lansaat; Ron van der Brink; Josep Arnabat-Dominguez; Lisette van der Molen; Irene Jacobi; Judi van Diessen; Jan de Lange; Ludi E Smeele; Mark M Schubert; René-Jean Bensadoun Journal: Support Care Cancer Date: 2016-03-16 Impact factor: 3.603
Authors: Cynthia M França; Cristiane M França; Silvia C Núñez; Renato A Prates; Elisângela Noborikawa; Miriam R Faria; Martha S Ribeiro Journal: J Photochem Photobiol B Date: 2008-09-30 Impact factor: 6.252
Authors: Noor Al-Dasooqi; Stephen T Sonis; Joanne M Bowen; Emma Bateman; Nicole Blijlevens; Rachel J Gibson; Richard M Logan; Raj G Nair; Andrea M Stringer; Roger Yazbeck; Sharon Elad; Rajesh V Lalla Journal: Support Care Cancer Date: 2013-07-11 Impact factor: 3.603
Authors: Rajesh V Lalla; Joanne Bowen; Andrei Barasch; Linda Elting; Joel Epstein; Dorothy M Keefe; Deborah B McGuire; Cesar Migliorati; Ourania Nicolatou-Galitis; Douglas E Peterson; Judith E Raber-Durlacher; Stephen T Sonis; Sharon Elad Journal: Cancer Date: 2014-02-25 Impact factor: 6.860
Authors: Mariana Bitu Ramos-Pinto; Teresa Paula de Lima Gusmão; Jayr Schmidt-Filho; Graziella Chagas Jaguar; Manoela Domingues Martins; Fábio Abreu Alves Journal: Support Care Cancer Date: 2021-04-27 Impact factor: 3.603
Authors: Mara Luana Batista Severo; Stéfanie Thieme; Felipe Martins Silveira; Raquel Padilha Martins Tavares; Amanda Katarinny Goes Gonzaga; Silvana Maria Zucolotto; Aurigena Antunes de Araújo; Marco Antonio Trevizani Martins; Manoela Domingues Martins; Éricka Janine Dantas da Silveira Journal: Support Care Cancer Date: 2021-11-25 Impact factor: 3.603
Authors: Ather Adnan; Anna N Yaroslavsky; James D Carroll; Wayne Selting; Amy F Juliano; Wendy B London; Stephen T Sonis; Christine N Duncan; Nathaniel S Treister Journal: Front Oral Health Date: 2021-07-16