Literature DB >> 34816401

Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases.

Clóvis Moreira1, Gustavo Marçal Schmidt Garcia Moreira2, Rafael Amaral Donassolo1, Marcos Roberto Alves Ferreira1, Mariliana Luiza Ferreira Alves1, Rafael Rodrigues Rodrigues1, Fabricio Rochedo Conceição3.   

Abstract

This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by the seroneutralization assay in mice, thereby showing to be an excellent alternative to induce a protective immune response against clostridial diseases.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cell lysate supernatant; Clostridiosis; Inclusion bodies; Recombinant antigens; Recombinant vaccines

Mesh：

Substances：

Year:  2022        PMID： 34816401     DOI： 10.1007/978-1-0716-1888-2_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Protective potential of recombinant non-purified botulinum neurotoxin serotypes C and D.

Authors:  Clóvis Moreira; Carlos Eduardo Pouey da Cunha; Gustavo Marçal Schmidt Garcia Moreira; Marcelo Mendonça; Felipe Masiero Salvarani; Ângela Nunes Moreira; Fabricio Rochedo Conceição
Journal:  Anaerobe       Date:  2016-05-25       Impact factor: 3.331

2.  Genetic characteristics of toxigenic Clostridia and toxin gene evolution.

Authors:  Michel R Popoff; Philippe Bouvet
Journal:  Toxicon       Date:  2013-05-23       Impact factor: 3.033

3.  Vaccination of cattle with a recombinant bivalent toxoid against botulism serotypes C and D.

Authors:  Carlos E P Cunha; Gustavo M S G Moreira; Felipe M Salvarani; Monique S Neves; Francisco C F Lobato; Odir A Dellagostin; Fabricio R Conceição
Journal:  Vaccine       Date:  2013-11-16       Impact factor: 3.641

4.  Production and evaluation of a recombinant chimeric vaccine against clostridium botulinum neurotoxin types C and D.

Authors:  Luciana A F Gil; Carlos Eduardo P da Cunha; Gustavo M S G Moreira; Felipe M Salvarani; Ronnie A Assis; Francisco Carlos F Lobato; Marcelo Mendonça; Odir A Dellagostin; Fabricio R Conceição
Journal:  PLoS One       Date:  2013-07-31       Impact factor: 3.240

5.  Immunogenicity of a Trivalent Recombinant Vaccine Against Clostridium perfringens Alpha, Beta, and Epsilon Toxins in Farm Ruminants.

Authors:  Gustavo Marçal Schmidt Garcia Moreira; Felipe Masiero Salvarani; Carlos Eduardo Pouey da Cunha; Marcelo Mendonça; Ângela Nunes Moreira; Luciana Aramuni Gonçalves; Prhiscylla Sadanã Pires; Francisco Carlos Faria Lobato; Fabricio Rochedo Conceição
Journal:  Sci Rep       Date:  2016-03-23       Impact factor: 4.379
  5 in total

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