| Literature DB >> 24252701 |
Carlos E P Cunha1, Gustavo M S G Moreira1, Felipe M Salvarani2, Monique S Neves2, Francisco C F Lobato2, Odir A Dellagostin1, Fabricio R Conceição3.
Abstract
Cattle botulism is a fatal intoxication caused by botulinum neurotoxins (BoNTs) produced by Clostridium botulinum serotypes C and D resulting in economic losses. Vaccination is the most effective way to control botulism. However, the commercially available vaccines are difficult and hazardous to produce. Neutralizing antibodies against the C-terminal fragment of the BoNT heavy chain (HC) are known to protect against lethal doses of BoNTs. We report the vaccination of cattle with a previously tested recombinant chimera consisting of Escherichia coli heat-labile enterotoxin B subunit and the HC of BoNTs C and D. Vaccinated animals produced neutralizing antibodies against serotypes C and D averaging 5±0 and 6.14±1.06IU/mL, respectively. For BoNT D, the titers were greater than those measured for the commercial vaccine, which induced titers of 5±0 and 2.85±1.35 against the respective serotypes, suggesting that this chimera is effective against cattle botulism.Entities:
Keywords: Botulinum neurotoxins; Clostridium botulinum; Fused antigens; LTB; Recombinant vaccine
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Year: 2013 PMID： 24252701 DOI： 10.1016/j.vaccine.2013.11.025
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641