Emre Aksoy1, Ali Tevfik Uncu2, Ertugrul Filiz3, Şule Orman4, Durmuş Çetin5, M Aydın Akbudak6. 1. Department of Biological Sciences, Middle East Technical University, Ankara, Turkey. 2. Department of Molecular Biology and Genetics, Necmettin Erbakan University, Konya, Turkey. 3. Department of Agricultural Production, Duzce University, Cilimli Vocational School, Duzce, Turkey. 4. Department of Soil Science and Plant Nutrition, Akdeniz University, Antalya, Turkey. 5. Department of Agricultural Biotechnology, Akdeniz University, Antalya, Turkey. 6. Department of Agricultural Biotechnology, Akdeniz University, Antalya, Turkey. akbudak@akdeniz.edu.tr.
Abstract
BACKGROUND: Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. METHODS AND RESULTS: In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins, heavy metal-associated proteins and NRAMP proteins. The Arabidopsis orthologs of the differentially expressed B. distachyon genes (DEGs) under Cd toxicity were identified, which exhibited Bradi4g26905 was an ortholog of AtALY1-2. Detailed co-expression network and gene ontology analyses found the potential involvement of the mRNA surveillance pathway in Cd tolerance in B. distachyon. These genes were shown to be downregulated by sulfur (S) deficiency. CONCLUSIONS: This is the first transcriptomic study investigating the effect of Cd toxicity in B. distachyon, a model plant for genomic studies in Poaceae (Gramineae) species. The results are expected to provide valuable information for more comprehensive research related to heavy metal toxicity in plants.
BACKGROUND: Cd accumulation in plant cells results in dramatic problems including oxidative stress and inhibition of vital enzymes. It also affects mineral uptakes by disrupting membrane permeability. Interaction among Cd and other plant nutrient elements changes the nutritional contents of crops and reduces their yield. METHODS AND RESULTS: In the present study, Cd stress in Brachypodium distachyon led to the upregulation of some heavy metal transport genes (influx or efflux) encoding cation-efflux proteins, heavy metal-associated proteins and NRAMP proteins. The Arabidopsis orthologs of the differentially expressed B. distachyon genes (DEGs) under Cd toxicity were identified, which exhibited Bradi4g26905 was an ortholog of AtALY1-2. Detailed co-expression network and gene ontology analyses found the potential involvement of the mRNA surveillance pathway in Cd tolerance in B. distachyon. These genes were shown to be downregulated by sulfur (S) deficiency. CONCLUSIONS: This is the first transcriptomic study investigating the effect of Cd toxicity in B. distachyon, a model plant for genomic studies in Poaceae (Gramineae) species. The results are expected to provide valuable information for more comprehensive research related to heavy metal toxicity in plants.
Authors: Sarah G Choudury; Saima Shahid; Diego Cuerda-Gil; Kaushik Panda; Alissa Cullen; Quratulayn Ashraf; Meredith J Sigman; Andrea D McCue; R Keith Slotkin Journal: Plant Cell Date: 2019-02-27 Impact factor: 11.277
Authors: Christina Pfaff; Hans F Ehrnsberger; María Flores-Tornero; Brian B Sørensen; Thomas Schubert; Gernot Längst; Joachim Griesenbeck; Stefanie Sprunck; Marion Grasser; Klaus D Grasser Journal: Plant Physiol Date: 2018-03-14 Impact factor: 8.340