Literature DB >> 3481263

Neutral endopeptidase-24.11 (enkephalinase). Biosynthesis and localization in human fibroblasts.

G Lorkowski1, J E Zijderhand-Bleekemolen, E G Erdös, K von Figura, A Hasilik.   

Abstract

The biosynthesis, glycosylation and subcellular localization of the neutral endopeptidase-24.11 were studied in cultured human fibroblasts. The enzyme was synthesized as a precursor (Mr 88,000) containing four or five N-linked oligosaccharides. Within 1 h the synthesis-mature (Mr 94,000) endopeptidase-24.11 was formed and contained sialylated oligosaccharides. The half-life of endopeptidase-24.11 was 3.7 days and in the presence of 10 mM-NH4Cl it increased to 6 days. Mature endopeptidase-24.11 was solubilized with 0.2% saponin and partitioned into Triton X-114. In intact fibroblasts, endopeptidase-24.11 was accessible to antibodies and to neuraminidase even when the treatment was performed at 4 degrees C. The localization of endopeptidase-24.11 to the plasma membrane in cultured fibroblasts was further demonstrated by immunocytochemistry.

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Year:  1987        PMID： 3481263      PMCID： PMC1148547          DOI： 10.1042/bj2480345

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  44 in total

1.  Mannose 6-phosphate-specific receptor is a transmembrane protein with a C-terminal extension oriented towards the cytosol.

Authors:  K von Figura; V Gieselmann; A Hasilik
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2.  An immunoradiometric assay for endopeptidase-24.11 shows it to be a widely distributed enzyme in pig tissues.

Authors:  N S Gee; M A Bowes; P Buck; A J Kenny
Journal:  Biochem J       Date:  1985-05-15       Impact factor: 3.857

3.  Processing and transport of lysosomal enzymes in human monocyte line U937.

Authors:  D K Gupta; A Schmidt; K von Figura; A Hasilik
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4.  Neutral metalloendopeptidase in human male genital tract. Comparison to angiotensin I-converting enzyme.

Authors:  E G Erdös; W W Schulz; J T Gafford; R Defendini
Journal:  Lab Invest       Date:  1985-04       Impact factor: 5.662

5.  The metabolism of neuropeptides. The hydrolysis of peptides, including enkephalins, tachykinins and their analogues, by endopeptidase-24.11.

Authors:  R Matsas; A J Kenny; A J Turner
Journal:  Biochem J       Date:  1984-10-15       Impact factor: 3.857

6.  Purification of endopeptidase-24.11 ('enkephalinase') from pig brain by immunoadsorbent chromatography.

Authors:  J M Relton; N S Gee; R Matsas; A J Turner; A J Kenny
Journal:  Biochem J       Date:  1983-12-01       Impact factor: 3.857

7.  Identification of a thermolysin-like metalloendopeptidase in serum: activity in normal subjects and in patients with sarcoidosis.

Authors:  J Almenoff; A S Teirstein; J C Thornton; M Orlowski
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8.  Proteins of the kidney microvillar membrane. Biosynthesis of endopeptidase-24.11, dipeptidylpeptidase IV and aminopeptidases N and A in pig kidney slices.

Authors:  J R Stewart; A J Kenny
Journal:  Biochem J       Date:  1984-12-01       Impact factor: 3.857

9.  Metabolism of neuropeptides. Hydrolysis of the angiotensins, bradykinin, substance P and oxytocin by pig kidney microvillar membranes.

Authors:  S L Stephenson; A J Kenny
Journal:  Biochem J       Date:  1987-01-01       Impact factor: 3.857

10.  Hydrolysis of substance p and neurotensin by converting enzyme and neutral endopeptidase.

Authors:  R A Skidgel; S Engelbrecht; A R Johnson; E G Erdös
Journal:  Peptides       Date:  1984 Jul-Aug       Impact factor: 3.750
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  16 in total

1.  Soluble enkephalin-degrading enzymes released by lymphomic and erythroleukaemic cell lines.

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3.  Fundamental insights into autosomal dominant polycystic kidney disease from human-based cell models.

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5.  Membrane peptidases on human osteoblast-like cells in culture: hydrolysis of calcitonin and hormonal regulation of endopeptidase-24.11.

Authors:  S Howell; A M Caswell; A J Kenny; A J Turner
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

6.  The proteoglycan perlecan is expressed in the erythroleukemia cell line K562 and is upregulated by sodium butyrate and phorbol ester.

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Journal:  Mol Cell Biochem       Date:  1995-04-12       Impact factor: 3.396

7.  Recombinant neutral endopeptidase-24.11 expressed in mouse neuroblastoma cells is associated with neurite membranes.

Authors:  G Lemay; M Zollinger; G Waksman; B P Roques; P Crine; G Boileau
Journal:  Biochem J       Date:  1990-04-15       Impact factor: 3.857

8.  Direct targeting of neutral endopeptidase (EC 3.4.24.11) to the apical cell surface of transfected LLC-PK1 cells and unpolarized secretion of its soluble form.

Authors:  C Lanctôt; H Fournier; S Howell; G Boileau; P Crine
Journal:  Biochem J       Date:  1995-01-01       Impact factor: 3.857

9.  Reduced circulating levels of angiotensin-(1--7) in systemic sclerosis: a new pathway in the dysregulation of endothelial-dependent vascular tone control.

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10.  Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity.

Authors:  C V Jongeneel; E J Quackenbush; P Ronco; P Verroust; S Carrel; M Letarte
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