| Literature DB >> 34811487 |
Yu-Feng Hu1,2,3, An-Sheng Lee4, Shih-Lin Chang5,6, Shien-Fong Lin7, Ching-Hui Weng5, Hsin-Yu Lo8, Pei-Chun Chou5, Yung-Nan Tsai5, Yen-Ling Sung7, Chien-Chang Chen9, Ruey-Bing Yang9, Yuh-Charn Lin9, Terry B J Kuo10, Cheng-Han Wu10, Jin-Dian Liu5, Tze-Wen Chung11,12, Shih-Ann Chen5,6,13.
Abstract
Pacemaker cells can be differentiated from stem cells or transdifferentiated from quiescent mature cardiac cells via genetic manipulation. Here we show that the exposure of rat quiescent ventricular cardiomyocytes to a silk-fibroin hydrogel activates the direct conversion of the quiescent cardiomyocytes to pacemaker cardiomyocytes by inducing the ectopic expression of the vascular endothelial cell-adhesion glycoprotein cadherin. The silk-fibroin-induced pacemaker cells exhibited functional and morphological features of genuine sinoatrial-node cardiomyocytes in vitro, and pacemaker cells generated via the injection of silk fibroin in the left ventricles of rats functioned as a surrogate in situ sinoatrial node. Biomaterials with suitable surface structure, mechanics and biochemistry could facilitate the scalable production of biological pacemakers for human use.Entities:
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Year: 2021 PMID： 34811487 DOI： 10.1038/s41551-021-00812-y
Source DB: PubMed Journal: Nat Biomed Eng ISSN： 2157-846X Impact factor: 29.234