Wei Yin Vinnie-Siow1, Tiong Kai Tan1, Van Lun Low2, Yong Bin Teoh3, Batah Kunalan Prakash4, Sinnadurai Sivanandam1, Reuben Sunil Kumar Sharma5, Yvonne Ai-Lian Lim6. 1. Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. 2. Higher Institution Centre of Excellence (HICoE), Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, Kuala Lumpur, Malaysia. vanlun_low@um.edu.my. 3. Laboratory of Veterinary Internal Medicine, Department of Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan. 4. Institute Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia. 5. Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Selangor, Malaysia. 6. Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. limailian@um.edu.my.
Abstract
PURPOSE: Canine filariosis in domestic dogs caused by several species of filarids is an emerging vector-borne disease and the spread of this disease remains a global veterinary and public health concern. However, information regarding these filarids and their epidemiological patterns remains scarce in Malaysia. The present study aimed to determine the infection rate and associated risk factors of filarial parasites in dogs in Malaysia. METHODS: A total of 399 dog blood samples were collected from veterinary hospitals and animal shelters in Malaysia to determine the infection rate and associated risk factors via a combination of microscopic, serologic and molecular diagnostic techniques. RESULTS: Two species of canine filariae identified in this study were Dirofilaria immitis (6.5%) and Brugia pahangi (1.3%), and their infections were associated with cross breed, medium size and short hair (p < 0.05). CONCLUSIONS: A new pair of primers was developed to complement the recovery of the 12S rRNA gene fragment of filarial parasites. This study represents the first molecular evidence of B. pahangi in dogs in Malaysia.
PURPOSE: Canine filariosis in domestic dogs caused by several species of filarids is an emerging vector-borne disease and the spread of this disease remains a global veterinary and public health concern. However, information regarding these filarids and their epidemiological patterns remains scarce in Malaysia. The present study aimed to determine the infection rate and associated risk factors of filarial parasites in dogs in Malaysia. METHODS: A total of 399 dog blood samples were collected from veterinary hospitals and animal shelters in Malaysia to determine the infection rate and associated risk factors via a combination of microscopic, serologic and molecular diagnostic techniques. RESULTS: Two species of canine filariae identified in this study were Dirofilaria immitis (6.5%) and Brugia pahangi (1.3%), and their infections were associated with cross breed, medium size and short hair (p < 0.05). CONCLUSIONS: A new pair of primers was developed to complement the recovery of the 12S rRNA gene fragment of filarial parasites. This study represents the first molecular evidence of B. pahangi in dogs in Malaysia.
Authors: Fernando Simón; Mar Siles-Lucas; Rodrigo Morchón; Javier González-Miguel; Isabel Mellado; Elena Carretón; Jose Alberto Montoya-Alonso Journal: Clin Microbiol Rev Date: 2012-07 Impact factor: 26.132
Authors: Claudio Genchi; Michele Mortarino; Laura Rinaldi; Giuseppe Cringoli; Giorgio Traldi; Marco Genchi Journal: Vet Parasitol Date: 2011-01-19 Impact factor: 2.738