| Literature DB >> 34791456 |
Pan Gong1,2, Michiel Bontinck1,2, Kirin Demuynck1,2, Jolien De Block1,2, Kris Gevaert3,4, Dominique Eeckhout1,2, Geert Persiau1,2, Stijn Aesaert1,2, Griet Coussens1,2, Mieke Van Lijsebettens1,2, Laurens Pauwels1,2, Geert De Jaeger1,2, Dirk Inzé1,2, Hilde Nelissen1,2.
Abstract
SAMBA has been identified as a plant-specific regulator of the anaphase-promoting complex/cyclosome (APC/C) that controls unidirectional cell cycle progression in Arabidopsis (Arabidopsis thaliana), but so far its role has not been studied in monocots. Here, we show the association of SAMBA with the APC/C is conserved in maize (Zea mays). Two samba genome edited mutants showed growth defects, such as reduced internode length, shortened upper leaves with erect leaf architecture, and reduced leaf size due to an altered cell division rate and cell expansion, which aggravated with plant age. The two mutants differed in the severity and developmental onset of the phenotypes, because samba-1 represented a knockout allele, while translation re-initiation in samba-3 resulted in a truncated protein that was still able to interact with the APC/C and regulate its function, albeit with altered APC/C activity and efficiency. Our data are consistent with a dosage-dependent role for SAMBA to control developmental processes for which a change in growth rate is pivotal. © American Society of Plant Biologists 2021. All rights reserved. For permissions, please email: journals.permissions@oup.com.Entities:
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Year: 2022 PMID： 34791456 PMCID： PMC8774815 DOI： 10.1093/plphys/kiab514
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340