Literature DB >> 3474613

Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumor antibiotic of Streptomyces glaucescens.

H Motamedi, C R Hutchinson.   

Abstract

Through complementation of mutations specifically blocking the biosynthesis of tetracenomycin C by Streptomyces glaucescens and selecting for resistance to tetracenomycin C in Streptomyces lividans, all of the genes for the production of tetracenomycin C were inserted in pIJ702, a high copy-number Streptomyces gene cloning vector. The tcm biosynthetic and resistance genes occur as a single cluster in the S. glaucescens genome and are expressed in heterologous streptomycete hosts like S. lividans, resulting in the overproduction of pigmented intermediates of the tetracenomycin C biosynthetic pathway.

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Year:  1987        PMID： 3474613      PMCID： PMC305106          DOI： 10.1073/pnas.84.13.4445

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Genetic analysis in a melanin-producing streptomycete, Streptomyces glaucescens.

Authors:  R Baumann; R Hütter; D A Hopwood
Journal:  J Gen Microbiol       Date:  1974-04

3.  Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2).

Authors:  B A Rudd; D A Hopwood
Journal:  J Gen Microbiol       Date:  1979-09

4.  DNAGEL: a computer program for determining DNA fragment sizes using a small computer equipped with a graphics tablet.

Authors:  T Kieser
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

5.  Adrenergic and cholinergic receptors of cerebral microvessels.

Authors:  S I Harik; V K Sharma; J R Wetherbee; R H Warren; S P Banerjee
Journal:  J Cereb Blood Flow Metab       Date:  1981       Impact factor: 6.200

6.  Scientific evidence on the role of Ayurvedic herbals on bioavailability of drugs.

Authors:  C K Atal; U Zutshi; P G Rao
Journal:  J Ethnopharmacol       Date:  1981-09       Impact factor: 4.360

7.  Homology between Streptomyces genes coding for synthesis of different polyketides used to clone antibiotic biosynthetic genes.

Authors:  F Malpartida; S E Hallam; H M Kieser; H Motamedi; C R Hutchinson; M J Butler; D A Sugden; M Warren; C McKillop; C R Bailey
Journal:  Nature       Date:  1987 Feb 26-Mar 4       Impact factor: 49.962

8.  [Metabolic products of microorganisms. 175. Tetracenomycin C (author's transl)].

Authors:  W Weber; H Zähner; J Siebers; K Schröder; A Zeeck
Journal:  Arch Microbiol       Date:  1979-05       Impact factor: 2.552

9.  Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.

Authors:  D A Hopwood; T Kieser; H M Wright; M J Bibb
Journal:  J Gen Microbiol       Date:  1983-07

10.  Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.

Authors:  E Katz; C J Thompson; D A Hopwood
Journal:  J Gen Microbiol       Date:  1983-09
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  29 in total

1.  An Oxetane-Based Polyketide Surrogate To Probe Substrate Binding in a Polyketide Synthase.

Authors:  Bryan D Ellis; Jacob C Milligan; Alexander R White; Vy Duong; Pilar X Altman; Lina Y Mohammed; Matthew P Crump; John Crosby; Ray Luo; Christopher D Vanderwal; Shiou-Chuan Tsai
Journal:  J Am Chem Soc       Date:  2018-04-10       Impact factor: 15.419

2.  The Streptomyces glaucescens TcmR protein represses transcription of the divergently oriented tcmR and tcmA genes by binding to an intergenic operator region.

Authors:  P G Guilfoile; C R Hutchinson
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

3.  Sequence and transcriptional analysis of the Streptomyces glaucescens tcmAR tetracenomycin C resistance and repressor gene loci.

Authors:  P G Guilfoile; C R Hutchinson
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

Review 4.  Streptomyces cloning: possible construction of novel compounds and regulation of antibiotic biosynthetic genes.

Authors:  P K Tomich
Journal:  Antimicrob Agents Chemother       Date:  1988-10       Impact factor: 5.191

Review 5.  The impact of genetic engineering on the commercial production of antibiotics by Streptomyces and related bacteria.

Authors:  C R Hutchinson
Journal:  Appl Biochem Biotechnol       Date:  1987 Sep-Dec       Impact factor: 2.926

6.  Cloning of spiramycin biosynthetic genes and their use in constructing Streptomyces ambofaciens mutants defective in spiramycin biosynthesis.

Authors:  M A Richardson; S Kuhstoss; M L Huber; L Ford; O Godfrey; J R Turner; R N Rao
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

7.  Multigene families for anthracycline antibiotic production in Streptomyces peucetius.

Authors:  K J Stutzman-Engwall; C R Hutchinson
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

8.  Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase.

Authors:  R G Summers; E Wendt-Pienkowski; H Motamedi; C R Hutchinson
Journal:  J Bacteriol       Date:  1992-03       Impact factor: 3.490

9.  Cloning and expression of daunorubicin biosynthesis genes from Streptomyces peucetius and S. peucetius subsp. caesius.

Authors:  S L Otten; K J Stutzman-Engwall; C R Hutchinson
Journal:  J Bacteriol       Date:  1990-06       Impact factor: 3.490

10.  Mutation and mapping of genes involved in production of the antibiotic TA in Myxococcus xanthus.

Authors:  M Varon; N Fuchs; M Monosov; S Tolchinsky; E Rosenberg
Journal:  Antimicrob Agents Chemother       Date:  1992-10       Impact factor: 5.191
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