Haile Tazeze1, Solomon Mequanente2, Dereje Nigussie3, Belete Legesse4, Eyasu Makonnen2,4, Teklie Mengie5. 1. Department of Pharmacy, Kidus Petros TB Specialized Hospital, Addis Ababa, Ethiopia. 2. Department of Pharmacology & Clinical Pharmacy, School of Pharmacy, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia. 3. Ethiopian Public Health Institute, Addis Ababa, Ethiopia. 4. Center for Innovative Drug Development & Therapeutics Trial in Africa (CDT-Africa), College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia. 5. Department of Pharmacy, College of Health Science, Debre Tabor University, Debre Tabor, Ethiopia.
Abstract
BACKGROUND: Traditionally Aloe trigonantha leaf is used for the treatment of different diseases. However, there were no in vivo studies which prove its claimed use for wound healing and anti-inflammatory activity. Therefore, the present study aimed at evaluating the in vivo wound healing and anti-inflammatory effects of the leaf gel of the plant in rats. METHODS: The leaf gel powder of the Aloe trigonantha was prepared after the gel gets lyophilized. It was evaluated for wound healing activity topically by incorporating it in a simple ointment base at a concentration of 5% (w/w) and 10% (w/w). Excision and incision models were used for wound healing activity in rats. For the excision wound model, wound contraction and period of epithelialization were evaluated, while wound tensile strength was evaluated using an incision wound model. A Xylene-induced ear edema model and cotton pellet-induced granuloma model were used for anti-inflammatory study. The leaf gel powder of Aloe trigonantha was given orally at a dose of 100, 200, and 400 mg/kg in both models of anti-inflammatory studies. An anti-inflammatory effect was measured by reduction of ear edema weight and reduction of wet exudate and dry granuloma weight in both of xylene-induced ear edema and cotton pellet-induced granuloma models, respectively. RESULTS: Treatment of wounds with ointment containing 5% and 10% (w/w) of the gel exhibited a significantly increased wound contraction rate, shorter epithelialization time, and higher skin breaking strength (p<0.05) compared to controls. Aloe trigonantha leaf gel powder also produced dose-dependent significant reductions (p<0.05) of inflammation compared to control in both models. CONCLUSION: Data obtained from this study collectively indicated that Aloe trigonantha is a potential wound-healing and anti-inflammatory agent in rat models of wound and inflammation which provides evidence for the traditional claim.
BACKGROUND: Traditionally Aloe trigonantha leaf is used for the treatment of different diseases. However, there were no in vivo studies which prove its claimed use for wound healing and anti-inflammatory activity. Therefore, the present study aimed at evaluating the in vivo wound healing and anti-inflammatory effects of the leaf gel of the plant in rats. METHODS: The leaf gel powder of the Aloe trigonantha was prepared after the gel gets lyophilized. It was evaluated for wound healing activity topically by incorporating it in a simple ointment base at a concentration of 5% (w/w) and 10% (w/w). Excision and incision models were used for wound healing activity in rats. For the excision wound model, wound contraction and period of epithelialization were evaluated, while wound tensile strength was evaluated using an incision wound model. A Xylene-induced ear edema model and cotton pellet-induced granuloma model were used for anti-inflammatory study. The leaf gel powder of Aloe trigonantha was given orally at a dose of 100, 200, and 400 mg/kg in both models of anti-inflammatory studies. An anti-inflammatory effect was measured by reduction of ear edema weight and reduction of wet exudate and dry granuloma weight in both of xylene-induced ear edema and cotton pellet-induced granuloma models, respectively. RESULTS: Treatment of wounds with ointment containing 5% and 10% (w/w) of the gel exhibited a significantly increased wound contraction rate, shorter epithelialization time, and higher skin breaking strength (p<0.05) compared to controls. Aloe trigonantha leaf gel powder also produced dose-dependent significant reductions (p<0.05) of inflammation compared to control in both models. CONCLUSION: Data obtained from this study collectively indicated that Aloe trigonantha is a potential wound-healing and anti-inflammatory agent in rat models of wound and inflammation which provides evidence for the traditional claim.
Authors: Dnyaneshwar D Kokane; Rahul Y More; Mandar B Kale; Minakshi N Nehete; Prachi C Mehendale; Chhaya H Gadgoli Journal: J Ethnopharmacol Date: 2009-05-03 Impact factor: 4.360