Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Repressible Promoter System to Study Essential Genes in Mycobacteria.

Literature DB >> 34709624

Repressible Promoter System to Study Essential Genes in Mycobacteria.

Francesca Boldrin¹, Riccardo Manganelli².

Abstract

Inducible gene expression systems represent powerful tools for studying essential gene function and for validation of drug targets in bacteria. Even if several regulated promoters have been characterized, only a few of them have been successfully used in Mycobacteria. Here we describe a successful mycobacterial gene regulation system based on the presence of two chromosomally encoded repressors: Pip and TetR, and a tunable promoter (Pptr) that allows a tight regulation of gene expression.

Entities: Chemical

Keywords: Conditional mutant; Essential gene; Gene expression; Mycobacteria; Repressible promoter; TetR/Pip-OFF

Mesh：

Substances：
Transcription Factors

Year: 2022 PMID： 34709624 DOI： 10.1007/978-1-0716-1720-5_17

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

Keyword Cloud
References

11 in total

1. Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.

Authors: Alessandro Cascioferro; Francesca Boldrin; Agnese Serafini; Roberta Provvedi; Giorgio Palù; Riccardo Manganelli
Journal: Appl Environ Microbiol Date: 2010-06-11 Impact factor: 4.792

2. The essential role of SepF in mycobacterial division.

Authors: Susanne Gola; Thomas Munder; Stefano Casonato; Riccardo Manganelli; Miguel Vicente
Journal: Mol Microbiol Date: 2015-06-06 Impact factor: 3.501

3. A Phenotypic Based Target Screening Approach Delivers New Antitubercular CTP Synthetase Inhibitors.

Authors: Marta Esposito; Sára Szadocka; Giulia Degiacomi; Beatrice S Orena; Giorgia Mori; Valentina Piano; Francesca Boldrin; Júlia Zemanová; Stanislav Huszár; David Barros; Sean Ekins; Joel Lelièvre; Riccardo Manganelli; Andrea Mattevi; Maria Rosalia Pasca; Giovanna Riccardi; Lluis Ballell; Katarína Mikušová; Laurent R Chiarelli
Journal: ACS Infect Dis Date: 2017-05-11 Impact factor: 5.084

4. The phosphatidyl-myo-inositol mannosyltransferase PimA is essential for Mycobacterium tuberculosis growth in vitro and in vivo.

Authors: Francesca Boldrin; Marcello Ventura; Giulia Degiacomi; Sudha Ravishankar; Claudia Sala; Zuzana Svetlikova; Anisha Ambady; Neeraj Dhar; Jana Kordulakova; Ming Zhang; Agnese Serafini; K G Vishwas; V G Vishwas; Gaëlle S Kolly; Naveen Kumar; Giorgio Palù; Marcelo E Guerin; Katarina Mikusova; Stewart T Cole; Riccardo Manganelli
Journal: J Bacteriol Date: 2014-07-21 Impact factor: 3.490

5. GarA is an essential regulator of metabolism in Mycobacterium tuberculosis.

Authors: Marcello Ventura; Barbara Rieck; Francesca Boldrin; Giulia Degiacomi; Marco Bellinzoni; Nathalie Barilone; Faisal Alzaidi; Pedro M Alzari; Riccardo Manganelli; Helen M O'Hare
Journal: Mol Microbiol Date: 2013-09-08 Impact factor: 3.501

6. Assessing the essentiality of the decaprenyl-phospho-d-arabinofuranose pathway in Mycobacterium tuberculosis using conditional mutants.

Authors: Gaëlle S Kolly; Francesca Boldrin; Claudia Sala; Neeraj Dhar; Ruben C Hartkoorn; Marcello Ventura; Agnese Serafini; John D McKinney; Riccardo Manganelli; Stewart T Cole
Journal: Mol Microbiol Date: 2014-03-07 Impact factor: 3.501

Repressible Promoter System to Study Essential Genes in Mycobacteria.

1. Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.

2. The essential role of SepF in mycobacterial division.

3. A Phenotypic Based Target Screening Approach Delivers New Antitubercular CTP Synthetase Inhibitors.

4. The phosphatidyl-myo-inositol mannosyltransferase PimA is essential for Mycobacterium tuberculosis growth in vitro and in vivo.

5. GarA is an essential regulator of metabolism in Mycobacterium tuberculosis.

6. Assessing the essentiality of the decaprenyl-phospho-d-arabinofuranose pathway in Mycobacterium tuberculosis using conditional mutants.

7. Conditional gene expression in Mycobacterium abscessus.

8. Essentiality of mmpL3 and impact of its silencing on Mycobacterium tuberculosis gene expression.

9. Improving the stability of the TetR/Pip-OFF mycobacterial repressible promoter system.

10. Promoter mutagenesis for fine-tuning expression of essential genes in Mycobacterium tuberculosis.