Literature DB >> 20543044

Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria.

Alessandro Cascioferro1, Francesca Boldrin, Agnese Serafini, Roberta Provvedi, Giorgio Palù, Riccardo Manganelli.   

Abstract

Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis.

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Year:  2010        PMID： 20543044      PMCID： PMC2916470          DOI： 10.1128/AEM.00382-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  Conservation of xer site-specific recombination genes in bacteria.

Authors:  G D Recchia; D J Sherratt
Journal:  Mol Microbiol       Date:  1999-12       Impact factor: 3.501

2.  Production of unmarked mutations in mycobacteria using site-specific recombination.

Authors:  Wladimir Malaga; Esther Perez; Christophe Guilhot
Journal:  FEMS Microbiol Lett       Date:  2003-02-28       Impact factor: 2.742

Review 3.  The TB epidemic from 1992 to 2002.

Authors:  Mario C Raviglione
Journal:  Tuberculosis (Edinb)       Date:  2003       Impact factor: 3.131

4.  Role of the extracytoplasmic-function sigma factor sigma(H) in Mycobacterium tuberculosis global gene expression.

Authors:  Riccardo Manganelli; Martin I Voskuil; Gary K Schoolnik; Eugenie Dubnau; Manuel Gomez; Issar Smith
Journal:  Mol Microbiol       Date:  2002-07       Impact factor: 3.501

5.  Development of a repressible mycobacterial promoter system based on two transcriptional repressors.

Authors:  Francesca Boldrin; Stefano Casonato; Elisa Dainese; Claudia Sala; Neeraj Dhar; Giorgio Palù; Giovanna Riccardi; Stewart T Cole; Riccardo Manganelli
Journal:  Nucleic Acids Res       Date:  2010-04-20       Impact factor: 16.971

6.  Rv1818c-encoded PE_PGRS protein of Mycobacterium tuberculosis is surface exposed and influences bacterial cell structure.

Authors:  Giovanni Delogu; Cinzia Pusceddu; Alessandra Bua; Giovanni Fadda; Michael J Brennan; Stefania Zanetti
Journal:  Mol Microbiol       Date:  2004-05       Impact factor: 3.501

7.  Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guérin.

Authors:  M H Lee; L Pascopella; W R Jacobs; G F Hatfull
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

8.  Expression and purification of recombinant methylated HBHA in Mycobacterium smegmatis.

Authors:  Giovanni Delogu; Alessandra Bua; Cinzia Pusceddu; Marcela Parra; Giovanni Fadda; Michael J Brennan; Stefania Zanetti
Journal:  FEMS Microbiol Lett       Date:  2004-10-01       Impact factor: 2.742

9.  Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis.

Authors:  Stoyan Bardarov; Svetoslav Bardarov; Martin S Pavelka; Vasan Sambandamurthy; Michelle Larsen; JoAnn Tufariello; John Chan; Graham Hatfull; William R Jacobs
Journal:  Microbiology       Date:  2002-10       Impact factor: 2.777

10.  Genes required for mycobacterial growth defined by high density mutagenesis.

Authors:  Christopher M Sassetti; Dana H Boyd; Eric J Rubin
Journal:  Mol Microbiol       Date:  2003-04       Impact factor: 3.501
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  19 in total

Review 1.  Gene replacement techniques for Escherichia coli genome modification.

Authors:  Mahesh Madyagol; Hend Al-Alami; Zdeno Levarski; Hana Drahovská; Ján Turňa; Stanislav Stuchlík
Journal:  Folia Microbiol (Praha)       Date:  2011-05-26       Impact factor: 2.099

2.  Assessment of Clofazimine and TB47 Combination Activity against Mycobacterium abscessus Using a Bioluminescent Approach.

Authors:  Yang Liu; Yaoju Tan; M Mahmudul Islam; Yuanyuan Cao; Xiaoyun Lu; Sheng Zeng; H M Adnan Hameed; Peipei Zhou; Xingshan Cai; Shuai Wang; Julius N Mugweru; Guoliang Zhang; Huancai Yin; Jianxiong Liu; Eric Nuermberger; Tianyu Zhang
Journal:  Antimicrob Agents Chemother       Date:  2020-02-21       Impact factor: 5.191

3.  Mycobacterium smegmatis MSMEG_3705 encodes a selective major facilitator superfamily efflux pump with multiple roles.

Authors:  Zhen Zhang; Rui Wang; Jianping Xie
Journal:  Curr Microbiol       Date:  2015-02-20       Impact factor: 2.188

4.  Repressible Promoter System to Study Essential Genes in Mycobacteria.

Authors:  Francesca Boldrin; Riccardo Manganelli
Journal:  Methods Mol Biol       Date:  2022

5.  Xer recombination for the automatic deletion of selectable marker genes from plasmids in enteric bacteria.

Authors:  Paola Salerno; Matthew W Leckenby; Bruce Humphrey; Rocky M Cranenburgh
Journal:  Synth Biol (Oxf)       Date:  2022-05-13

6.  pks5-recombination-mediated surface remodelling in Mycobacterium tuberculosis emergence.

Authors:  Eva C Boritsch; Wafa Frigui; Alessandro Cascioferro; Wladimir Malaga; Gilles Etienne; Françoise Laval; Alexandre Pawlik; Fabien Le Chevalier; Mickael Orgeur; Laurence Ma; Christiane Bouchier; Timothy P Stinear; Philip Supply; Laleh Majlessi; Mamadou Daffé; Christophe Guilhot; Roland Brosch
Journal:  Nat Microbiol       Date:  2016-01-27       Impact factor: 17.745

7.  The ESX-3 secretion system is necessary for iron and zinc homeostasis in Mycobacterium tuberculosis.

Authors:  Agnese Serafini; Davide Pisu; Giorgio Palù; G Marcela Rodriguez; Riccardo Manganelli
Journal:  PLoS One       Date:  2013-10-14       Impact factor: 3.240

8.  Engineering more stable, selectable marker-free autoluminescent mycobacteria by one step.

Authors:  Feng Yang; Moses M Njire; Jia Liu; Tian Wu; Bangxing Wang; Tianzhou Liu; Yuanyuan Cao; Zhiyong Liu; Junting Wan; Zhengchao Tu; Yaoju Tan; Shouyong Tan; Tianyu Zhang
Journal:  PLoS One       Date:  2015-03-11       Impact factor: 3.240

9.  Efficient and simple generation of multiple unmarked gene deletions in Mycobacterium smegmatis.

Authors:  Xu-Jian Mao; Mei-Yi Yan; Hui Zhu; Xiao-Peng Guo; Yi-Cheng Sun
Journal:  Sci Rep       Date:  2016-03-14       Impact factor: 4.379

10.  Mycobacterium tuberculosis Rv1152 is a Novel GntR Family Transcriptional Regulator Involved in Intrinsic Vancomycin Resistance and is a Potential Vancomycin Adjuvant Target.

Authors:  Jie Zeng; Wanyan Deng; Wenmin Yang; Hongping Luo; Xiangke Duan; Longxiang Xie; Ping Li; Rui Wang; Tiwei Fu; Abualgasim Elgaili Abdalla; Jianping Xie
Journal:  Sci Rep       Date:  2016-06-28       Impact factor: 4.379
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