Literature DB >> 3470007

Specific A . T DNA sequence binding of RP-HPLC purified HMG-I.

T S Elton, M S Nissen, R Reeves.   

Abstract

HMG-I (alpha-protein) is a high mobility group protein which recognizes and binds specifically to A . T rich double stranded DNA. We have investigated, by electrophoretic shift assays and DNase I footprinting, the ability of reverse-phase high performance liquid chromatography purified HMG-I to bind to specific A . T rich duplex DNA sequences. We show here that when HMG-I is isolated and purified under denaturing conditions it retains its specific A . T DNA binding activity. These results suggest that reverse-phase high performance liquid chromatography to be the method of choice for the preparation of HMG-I.

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Year:  1987        PMID： 3470007     DOI： 10.1016/0006-291x(87)90659-0

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  17 in total

1.  The amino terminus of Epstein-Barr Virus (EBV) nuclear antigen 1 contains AT hooks that facilitate the replication and partitioning of latent EBV genomes by tethering them to cellular chromosomes.

Authors:  John Sears; Maki Ujihara; Samantha Wong; Christopher Ott; Jaap Middeldorp; Ashok Aiyar
Journal:  J Virol       Date:  2004-11       Impact factor: 5.103

2.  A poly(dA-dT) upstream activating sequence binds high-mobility group I protein and contributes to lymphotoxin (tumor necrosis factor-beta) gene regulation.

Authors:  S J Fashena; R Reeves; N H Ruddle
Journal:  Mol Cell Biol       Date:  1992-02       Impact factor: 4.272

3.  HMG I-like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters.

Authors:  K Jacobsen; N B Laursen; E O Jensen; A Marcker; C Poulsen; K A Marcker
Journal:  Plant Cell       Date:  1990-01       Impact factor: 11.277

4.  Purification and characterization of a high-mobility-group-like DNA-binding protein that stimulates rRNA synthesis in vitro.

Authors:  H F Yang-Yen; L I Rothblum
Journal:  Mol Cell Biol       Date:  1988-08       Impact factor: 4.272

5.  Protein phosphatase 2A1 is the major enzyme in vertebrate cell extracts that dephosphorylates several physiological substrates for cyclin-dependent protein kinases.

Authors:  P Ferrigno; T A Langan; P Cohen
Journal:  Mol Biol Cell       Date:  1993-07       Impact factor: 4.138

6.  Inhibition of HMGI-C protein synthesis suppresses retrovirally induced neoplastic transformation of rat thyroid cells.

Authors:  M T Berlingieri; G Manfioletti; M Santoro; A Bandiera; R Visconti; V Giancotti; A Fusco
Journal:  Mol Cell Biol       Date:  1995-03       Impact factor: 4.272

7.  Calcium-dependent ADP-ribosylation of high-mobility-group I (HMGI) proteins.

Authors:  V Giancotti; A Bandiera; C Sindici; L Perissin; C Crane-Robinson
Journal:  Biochem J       Date:  1996-08-01       Impact factor: 3.857

8.  HMGI(Y) and Sp1 in addition to NF-kappa B regulate transcription of the MGSA/GRO alpha gene.

Authors:  L D Wood; A A Farmer; A Richmond
Journal:  Nucleic Acids Res       Date:  1995-10-25       Impact factor: 16.971

9.  Cux/CDP homeoprotein is a component of NF-muNR and represses the immunoglobulin heavy chain intronic enhancer by antagonizing the bright transcription activator.

Authors:  Z Wang; A Goldstein; R T Zong; D Lin; E J Neufeld; R H Scheuermann; P W Tucker
Journal:  Mol Cell Biol       Date:  1999-01       Impact factor: 4.272

10.  Macronuclei and micronuclei in Tetrahymena thermophila contain high-mobility-group-like chromosomal proteins containing a highly conserved eleven-amino-acid putative DNA-binding sequence.

Authors:  I G Schulman; T Wang; M Wu; J Bowen; R G Cook; M A Gorovsky; C D Allis
Journal:  Mol Cell Biol       Date:  1991-01       Impact factor: 4.272
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