| Literature DB >> 34694621 |
Tennille Sibbritt1, Ulrike Schumann1, Andrew Shafik1, Marco Guarnacci1, Susan J Clark2,3, Thomas Preiss4,5.
Abstract
Mapping the position and quantifying the level of 5-methylcytosine (m5C) as a modification in different types of cellular RNA is an important objective in the field of epitranscriptomics. Bisulfite conversion has long been the gold standard for the detection of m5C in DNA, but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification, and detection of candidate sites by sequencing on the Illumina MiSeq platform.Entities:
Keywords: 5-methylcytosine; Bisulfite conversion; Epitranscriptomics; MiSeq; Next-generation sequencing
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Year: 2022 PMID： 34694621 DOI： 10.1007/978-1-0716-1851-6_21
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745