Literature DB >> 34694611

Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH).

Andrea Zeni1,2, Margherita Grasso1,3, Michela A Denti4.   

Abstract

microRNA capture affinity technology (miR-CATCH) uses affinity capture biotinylated antisense oligonucleotides to co-purify a target transcript together with all its endogenously bound miRNAs. The miR-CATCH assay is performed to investigate miRNAs bound to a specific mRNA. This method allows to have a total vision of miRNAs bound not only to the 3'UTR but also to the 5'UTR and Coding Region of target messenger RNAs (mRNAs).
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Post-transcriptional regulation; Pull-down; miR-CATCH; miRNA target analysis; miRNAs

Mesh：

Substances：

Year:  2022        PMID： 34694611     DOI： 10.1007/978-1-0716-1851-6_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

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3.  Systematic evaluation of the microRNAome through miR-CATCHv2.0 identifies positive and negative regulators of BRAF-X1 mRNA.

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Journal:  RNA Biol       Date:  2019-04-19       Impact factor: 4.652

4.  CircNAPEPLD is expressed in human and murine spermatozoa and physically interacts with oocyte miRNAs.

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Journal:  RNA Biol       Date:  2019-06-14       Impact factor: 4.652

5.  Unipro UGENE: a unified bioinformatics toolkit.

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Journal:  Bioinformatics       Date:  2012-02-24       Impact factor: 6.937

6.  Thermodynamics-structure relationship of single mismatches in RNA/DNA duplexes.

Authors:  N Sugimoto; M Nakano; S Nakano
Journal:  Biochemistry       Date:  2000-09-19       Impact factor: 3.162

7.  miR-CATCH Identifies Biologically Active miRNA Regulators of the Pro-Survival Gene XIAP, in Chinese Hamster Ovary Cells.

Authors:  Alan Griffith; Paul S Kelly; Sebastian Vencken; Nga T Lao; Catherine M Greene; Martin Clynes; Niall Barron
Journal:  Biotechnol J       Date:  2017-10-30       Impact factor: 4.677

8.  Identification of MiR-21-5p as a Functional Regulator of Mesothelin Expression Using MicroRNA Capture Affinity Coupled with Next Generation Sequencing.

Authors:  Chiara De Santi; Sebastian Vencken; Jonathon Blake; Bettina Haase; Vladimir Benes; Federica Gemignani; Stefano Landi; Catherine M Greene
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9.  Reduced miR-659-3p Levels Correlate with Progranulin Increase in Hypoxic Conditions: Implications for Frontotemporal Dementia.

Authors:  Paola Piscopo; Margherita Grasso; Francesca Fontana; Alessio Crestini; Maria Puopolo; Valerio Del Vescovo; Aldina Venerosi; Gemma Calamandrei; Sebastian F Vencken; Catherine M Greene; Annamaria Confaloni; Michela A Denti
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Journal:  Sci Rep       Date:  2016-08-16       Impact factor: 4.379
  10 in total

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