Literature DB >> 3468946

In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation.

R Narayanan, M M Jastreboff, C F Chiu, J R Bertino.   

Abstract

Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.

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Year:  1986        PMID: 3468946     DOI: 10.1016/s0006-291x(86)80146-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN: 0006-291X            Impact factor:   3.575


  6 in total

1.  Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.

Authors:  D J Winterbourne; S Thomas; J Hermon-Taylor; I Hussain; A P Johnstone
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

2.  Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells.

Authors:  F Toneguzzo; A Keating; S Glynn; K McDonald
Journal:  Nucleic Acids Res       Date:  1988-06-24       Impact factor: 16.971

3.  Neoplastic transformation of mouse mammary epithelial cells by deregulated myc expression.

Authors:  N T Telang; M P Osborne; L A Sweterlitsch; R Narayanan
Journal:  Cell Regul       Date:  1990-10

4.  Induction of cervical neoplasia in the mouse by herpes simplex virus type 2 DNA.

Authors:  D D Anthony; W B Wentz; J W Reagan; A D Heggie
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

5.  Transcriptional regulation of the rat platelet factor 4 gene: interaction between an enhancer/silencer domain and the GATA site.

Authors:  K Ravid; T Doi; D L Beeler; D J Kuter; R D Rosenberg
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272

6.  Effect of chemokine receptor CXCR4 on hypoxia-induced pulmonary hypertension and vascular remodeling in rats.

Authors:  Lunyin Yu; Charles A Hales
Journal:  Respir Res       Date:  2011-02-04
  6 in total

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