Literature DB >> 3401216

Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.

D J Winterbourne1, S Thomas, J Hermon-Taylor, I Hussain, A P Johnstone.   

Abstract

The effect of various parameters on the electric shock-mediated permeabilization and transfection of CHO cells has been investigated. Up to 70% of the cells can be maintained transiently permeable to erythrosin B for periods of at least 1 h at 20 degrees C. Electrical conditions optimal for transient permeabilization were also optimal for efficient DNA transfection by pSV2neo. However, the DNA must be present during exposure to the electric field for efficient transformation. The same requirement existed for voltage-induced DNA toxicity. The results suggest that DNA moves into the cells by electrophoresis, not by simple diffusion. Based on these observations a simple, rapid procedure for optimizing the conditions for electric shock-mediated DNA transfer into cells has been developed.

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Year:  1988        PMID： 3401216      PMCID： PMC1149020          DOI： 10.1042/bj2510427

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Electric field mediated transformation: isolation and characterization of a TK+ subclone.

Authors:  D Zerbib; F Amalric; J Teissié
Journal:  Biochem Biophys Res Commun       Date:  1985-06-28       Impact factor: 3.575

2.  Changes in topoisomerase I activity after irradiation of lymphoid cells.

Authors:  A Johnstone; R McNerney
Journal:  Biosci Rep       Date:  1985 Oct-Nov       Impact factor: 3.840

3.  Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

Authors:  F T Kao; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1968-08       Impact factor: 11.205

4.  Induction of calcium-dependent, localized cortical granule breakdown in sea-urchin eggs by voltage pulsation.

Authors:  D P Rossignol; G L Decker; W J Lennarz; T Y Tsong; J Teissie
Journal:  Biochim Biophys Acta       Date:  1983-12-19

5.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors:  P J Southern; P Berg
Journal:  J Mol Appl Genet       Date:  1982

Review 6.  Electric field-induced cell-to-cell fusion.

Authors:  U Zimmermann; J Vienken
Journal:  J Membr Biol       Date:  1982       Impact factor: 1.843

7.  Voltage-induced pore formation and hemolysis of human erythrocytes.

Authors:  K Kinosita; T Y Tsong
Journal:  Biochim Biophys Acta       Date:  1977-12-01

8.  Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

Authors:  H Potter; L Weir; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

9.  Reversible and irreversible modification of erythrocyte membrane permeability by electric field.

Authors:  E H Serpersu; K Kinosita; T Y Tsong
Journal:  Biochim Biophys Acta       Date:  1985-02-14

10.  Gene transfer into mouse lyoma cells by electroporation in high electric fields.

Authors:  E Neumann; M Schaefer-Ridder; Y Wang; P H Hofschneider
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  14 in total

1.  Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.

Authors:  S I Sukharev; V A Klenchin; S M Serov; L V Chernomordik
Journal:  Biophys J       Date:  1992-11       Impact factor: 4.033

2.  Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis.

Authors:  V A Klenchin; S I Sukharev; S M Serov; L V Chernomordik
Journal:  Biophys J       Date:  1991-10       Impact factor: 4.033

Review 3.  Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 1- Biophysical mechanisms.

Authors:  Jean-Michel Escoffre; Chloé Mauroy; Thomas Portet; Luc Wasungu; Chrystelle Rosazza; Yoann Gilbart; Laetitia Mallet; Elisabeth Bellard; Muriel Golzio; Marie-Pierre Rols; Justin Teissié
Journal:  Biophys Rev       Date:  2009-11-17

4.  Control by pulse parameters of electric field-mediated gene transfer in mammalian cells.

Authors:  H Wolf; M P Rols; E Boldt; E Neumann; J Teissié
Journal:  Biophys J       Date:  1994-02       Impact factor: 4.033

5.  Electroporation technique of DNA transfection.

Authors:  S C Spencer
Journal:  Appl Biochem Biotechnol       Date:  1993-07       Impact factor: 2.926

6.  A recombinant virus assay using full-length envelope sequences to detect changes in HIV-1 co-receptor usage.

Authors:  M T Dittmar; S Eichler; S Reinberger; L Henning; H G Kräusslich
Journal:  Virus Genes       Date:  2001-12       Impact factor: 2.332

7.  Combined pulse electroporation--a novel strategy for highly efficient transfection of human and mouse cells.

Authors:  Thorsten Stroh; Ulrike Erben; Anja A Kühl; Martin Zeitz; Britta Siegmund
Journal:  PLoS One       Date:  2010-03-02       Impact factor: 3.240

8.  Transient gene expression in electroporated Solanum protoplasts.

Authors:  H Jones; G Ooms; M G Jones
Journal:  Plant Mol Biol       Date:  1989-11       Impact factor: 4.076

9.  Recombinant virus assay: a rapid, phenotypic assay for assessment of drug susceptibility of human immunodeficiency virus type 1 isolates.

Authors:  P Kellam; B A Larder
Journal:  Antimicrob Agents Chemother       Date:  1994-01       Impact factor: 5.191

10.  Modulation of gene activity by consecutive gene targeting of one creatine kinase M allele in mouse embryonic stem cells.

Authors:  J van Deursen; R Lovell-Badge; F Oerlemans; J Schepens; B Wieringa
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971
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