Literature DB >> 3467328

Rapid plasmid library screening using RecA-coated biotinylated probes.

B Rigas, A A Welcher, D C Ward, S M Weissman.   

Abstract

A method for the rapid physical isolation of recombinant plasmids of interest from a mixture of plasmids such as a plasmid cDNA library is presented. This method utilizes the ability of RecA protein to form stable complexes between linear single-stranded and circular double-stranded DNA molecules sharing sequence homology, and procedures allowing isolation of biotinylated nucleic acid. Biotinylated linear DNA probes coated with RecA have been used to screen reconstituted plasmid libraries consisting of two plasmid species, one homologous and the other heterologous to the probe. When the link between biotin and the nucleotide base could be cleaved by reducing agents, the complex was purified by streptavidin-agarose chromatography and the recovered plasmid was propagated in Escherichia coli. When the link was not cleavable the complex was bound to avidin in solution and purified by cupric iminodiacetic acid-agarose chromatography. The complex was then dissociated and the plasmids were propagated in E. coli. With either protocol, homologous plasmid recovery was between 10% and 20%, and enrichment was between 10(4)- and 10(5)-fold. Potential applications and extensions of this method, such as plasmid, cosmid, and phage library screening and facilitation of physical mapping of macroregions of mammalian genomes are presented and discussed.

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Year:  1986        PMID: 3467328      PMCID: PMC387186          DOI: 10.1073/pnas.83.24.9591

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  10 in total

1.  Affinity chromatography of DNA labeled with chemically cleavable biotinylated nucleotide analogs.

Authors:  T M Herman; E Lefever; M Shimkus
Journal:  Anal Biochem       Date:  1986-07       Impact factor: 3.365

Review 2.  Molecular organization of the class I genes of human major histocompatibility complex.

Authors:  R Srivastava; B W Duceman; P A Biro; A K Sood; S M Weissman
Journal:  Immunol Rev       Date:  1985-07       Impact factor: 12.988

Review 3.  Homologous pairing and strand exchange in genetic recombination.

Authors:  C M Radding
Journal:  Annu Rev Genet       Date:  1982       Impact factor: 16.830

4.  Role of superhelicity in homologous pairing of DNA molecules promoted by Escherichia coli recA protein.

Authors:  T Shibata; T Ohtani; P K Chang; T Ando
Journal:  J Biol Chem       Date:  1982-01-10       Impact factor: 5.157

5.  Human transforming growth factor-alpha: precursor structure and expression in E. coli.

Authors:  R Derynck; A B Roberts; M E Winkler; E Y Chen; D V Goeddel
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

6.  The pairing activity of stable nucleoprotein filaments made from recA protein, single-stranded DNA, and adenosine 5'-(gamma-thio)triphosphate.

Authors:  S M Honigberg; D K Gonda; J Flory; C M Radding
Journal:  J Biol Chem       Date:  1985-09-25       Impact factor: 5.157

7.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

8.  Synapsis and the formation of paranemic joints by E. coli RecA protein.

Authors:  M Bianchi; C DasGupta; C M Radding
Journal:  Cell       Date:  1983-10       Impact factor: 41.582

9.  recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.

Authors:  M M Cox; I R Lehman
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

10.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616

  10 in total
  13 in total

1.  Branch capture reactions: displacers derived from asymmetric PCR.

Authors:  D M Wong; P H Weinstock; J G Wetmur
Journal:  Nucleic Acids Res       Date:  1991-05-11       Impact factor: 16.971

2.  Triplex affinity capture of a single copy clone from a yeast genomic library.

Authors:  T Ito; C L Smith; C R Cantor
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Sequence-specific DNA purification by triplex affinity capture.

Authors:  T Ito; C L Smith; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-15       Impact factor: 11.205

4.  Preparation of a differentially expressed, full-length cDNA expression library by RecA-mediated triple-strand formation with subtractively enriched cDNA fragments.

Authors:  T B Hakvoort; J A Spijkers; J L Vermeulen; W H Lamers
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

5.  Use of RecA protein to enrich for homologous genes in a genomic library.

Authors:  B Taidi-Laskowski; D Tyan; S M Honigberg; C R Radding; F C Grumet
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

6.  Branch capture reactions: effect of recipient structure.

Authors:  P H Weinstock; J G Wetmur
Journal:  Nucleic Acids Res       Date:  1990-07-25       Impact factor: 16.971

7.  Sequence-specific ligation of DNA using RecA protein.

Authors:  L J Ferrin; R D Camerini-Otero
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-03       Impact factor: 11.205

8.  Sequence-specific targeting and covalent modification of human genomic DNA.

Authors:  E S Belousov; I A Afonina; M A Podyminogin; H B Gamper; M W Reed; R M Wydro; R B Meyer
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

9.  Solid phase DNA sequencing using the biotin-avidin system.

Authors:  S Ståhl; T Hultman; A Olsson; T Moks; M Uhlén
Journal:  Nucleic Acids Res       Date:  1988-04-11       Impact factor: 16.971

10.  Selective enrichment of a large size genomic DNA fragment by affinity capture: an approach for genome mapping.

Authors:  R P Kandpal; D C Ward; S M Weissman
Journal:  Nucleic Acids Res       Date:  1990-04-11       Impact factor: 16.971

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