Literature DB >> 3466162

Formation of functional asialoglycoprotein receptor after transfection with cDNAs encoding the receptor proteins.

M McPhaul, P Berg.   

Abstract

The rat asialoglycoprotein receptor (ASGP-R) has been expressed in cultured rat hepatoma cells (HTC cells) after transfection with cloned cDNAs. Fluorescence-activated cell sorting of transfected cells was used to identify the functional cDNA clones and to isolate cells expressing the ASGP-R. Simultaneous or sequential transfections with two cloned cDNAs that encode related but distinctive polypeptide chains were needed to obtain ASGP-R activity; transfection with either cDNA alone failed to produce detectable ASGP-R. The affinity of transduced ASGP-R for asialo orosomucoid is less than that of the native rat ASGP-R, and the number of surface receptors in clones expressing ASGP-R is about one-fifth that found on rat hepatocytes.

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Year:  1986        PMID： 3466162      PMCID： PMC387033          DOI： 10.1073/pnas.83.23.8863

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Chemical and physical properties of an hepatic membrane protein that specifically binds asialoglycoproteins.

Authors:  T Kawasaki; G Ashwell
Journal:  J Biol Chem       Date:  1976-03-10       Impact factor: 5.157

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Studies on a mammalian hepatic binding protein specific for asialoglycoproteins. Evidence for receptor recycling in isolated rat hepatocytes.

Authors:  C J Steer; G Ashwell
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

4.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

Review 5.  Carbohydrate-specific receptors of the liver.

Authors:  G Ashwell; J Harford
Journal:  Annu Rev Biochem       Date:  1982       Impact factor: 23.643

6.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors:  P J Southern; P Berg
Journal:  J Mol Appl Genet       Date:  1982

7.  A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum.

Authors:  M A Markwell
Journal:  Anal Biochem       Date:  1982-09-15       Impact factor: 3.365

8.  Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue.

Authors:  E Frost; J Williams
Journal:  Virology       Date:  1978-11       Impact factor: 3.616

9.  Immunological approaches to the study of membrane receptors. A monoclonal antibody that inhibits the binding of asialoglycoproteins to the rat liver receptor.

Authors:  J Harford; M Lowe; H Tsunoo; G Ashwell
Journal:  J Biol Chem       Date:  1982-11-10       Impact factor: 5.157

10.  Cell surface distribution and intracellular fate of asialoglycoproteins: a morphological and biochemical study of isolated rat hepatocytes and monolayer cultures.

Authors:  P L Zeitlin; A L Hubbard
Journal:  J Cell Biol       Date:  1982-03       Impact factor: 10.539
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  18 in total

1.  Assembly of a heterooligomeric asialoglycoprotein receptor complex during cell-free translation.

Authors:  J T Sawyer; D Doyle
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

2.  The two subunits of the human asialoglycoprotein receptor have different fates when expressed alone in fibroblasts.

Authors:  M A Shia; H F Lodish
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

3.  Identification and characterization of cDNA clones encoding two homologous proteins that are part of the asialoglycoprotein receptor.

Authors:  M McPhaul; P Berg
Journal:  Mol Cell Biol       Date:  1987-05       Impact factor: 4.272

4.  Isolation and characterization of cDNAs coding for the beta subunit of the high-affinity receptor for immunoglobulin E.

Authors:  J P Kinet; U Blank; C Ra; K White; H Metzger; J Kochan
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

5.  The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acid alpha 2,6GalNAc.

Authors:  Eric I Park; Yiling Mi; Carlo Unverzagt; Hans-Joachim Gabius; Jacques U Baenziger
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-14       Impact factor: 11.205

6.  Oligomeric structure of the human asialoglycoprotein receptor: nature and stoichiometry of mutual complexes containing H1 and H2 polypeptides assessed by fluorescence photobleaching recovery.

Authors:  Y I Henis; Z Katzir; M A Shia; H F Lodish
Journal:  J Cell Biol       Date:  1990-10       Impact factor: 10.539

7.  Rat liver beta-glucuronidase. cDNA cloning, sequence comparisons and expression of a chimeric protein in COS cells.

Authors:  P P Powell; J W Kyle; R D Miller; J Pantano; J H Grubb; W S Sly
Journal:  Biochem J       Date:  1988-03-01       Impact factor: 3.857

8.  A mammalian sperm lectin related to rat hepatocyte lectin-2/3. Purification from rabbit testis and identification as a zona binding protein.

Authors:  M Abdullah; E E Widgren; M G O'Rand
Journal:  Mol Cell Biochem       Date:  1991-05-15       Impact factor: 3.396

9.  Human homologue of mouse lymph node homing receptor: evolutionary conservation at tandem cell interaction domains.

Authors:  M H Siegelman; I L Weissman
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

10.  The differences in structural specificity for recognition and binding between asialoglycoprotein receptors of liver and macrophages.

Authors:  K Ozaki; R T Lee; Y C Lee; T Kawasaki
Journal:  Glycoconj J       Date:  1995-06       Impact factor: 2.916
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