Yunliang Lei1, Jiabin Peng1,2, Zhu Dai1, Ying Liao1, Quanhui Liu1, Jian Li1, Yonghui Jiang1. 1. Department of Orthopaedics, the First Affiliated Hospital of University of South China, Hengyang, Hunan, China. 2. Guangdong Hospital of Traditional Chinese Medicine, Zhuhai, Guangdong, China.
Abstract
OBJECTIVE: This study was undertaken to elucidate the mechanism of improved chondrocyte migration after juvenile articular cartilage fragmentation. DESIGN: In vitro organ culture with rabbit cartilage fragments and cell culture with rabbit chondrocytes were performed. In part A, minced juvenile cartilage fragments (~0.5 × 0.5 × 0.5 mm) from rabbits, planted in gelatin sponge and fibrin glue, were cultured for 2, 4, or 6 weeks in vitro and compared with the cartilage chunks (~4 × 4 × 1 mm) and membrane type 1 matrix metalloprotease (MT1-MMP) inhibitor groups. Chondrocyte outgrowth was evaluated on histology and confocal laser scanning microscopy. MT1-MMP expression was compared between the cartilage fragment group and the cartilage chunks group. In part B, articular chondrocytes were harvested from juvenile rabbits, MT1-MMP was transfected into the cells, and cell migration was evaluated using the Transwell and wound healing tests. RESULTS: The histology and confocal microscopy results revealed that cell accumulation occurred at the edge of cartilage fragments, and outgrowth was better in the cartilage fragment group than those in the cartilage chunks group. Similar results were observed for MT1-MMP expression. After MT1-MMP inhibition, cells did not accumulate at the edge of the cartilage fragments, and chondrocyte outgrowth did not occur. Furthermore, overexpression of MT1-MMP enhanced the migration of articular chondrocytes. CONCLUSIONS: Juvenile articular cartilage fragmentation improved chondrocyte migration by upregulating MT1-MMP.
OBJECTIVE: This study was undertaken to elucidate the mechanism of improved chondrocyte migration after juvenile articular cartilage fragmentation. DESIGN: In vitro organ culture with rabbit cartilage fragments and cell culture with rabbit chondrocytes were performed. In part A, minced juvenile cartilage fragments (~0.5 × 0.5 × 0.5 mm) from rabbits, planted in gelatin sponge and fibrin glue, were cultured for 2, 4, or 6 weeks in vitro and compared with the cartilage chunks (~4 × 4 × 1 mm) and membrane type 1 matrix metalloprotease (MT1-MMP) inhibitor groups. Chondrocyte outgrowth was evaluated on histology and confocal laser scanning microscopy. MT1-MMP expression was compared between the cartilage fragment group and the cartilage chunks group. In part B, articular chondrocytes were harvested from juvenile rabbits, MT1-MMP was transfected into the cells, and cell migration was evaluated using the Transwell and wound healing tests. RESULTS: The histology and confocal microscopy results revealed that cell accumulation occurred at the edge of cartilage fragments, and outgrowth was better in the cartilage fragment group than those in the cartilage chunks group. Similar results were observed for MT1-MMP expression. After MT1-MMP inhibition, cells did not accumulate at the edge of the cartilage fragments, and chondrocyte outgrowth did not occur. Furthermore, overexpression of MT1-MMP enhanced the migration of articular chondrocytes. CONCLUSIONS: Juvenile articular cartilage fragmentation improved chondrocyte migration by upregulating MT1-MMP.
Entities:
Keywords:
MT1-MMP; cartilage fragmentation; chondrocyte outgrowth; in vitro
Authors: H Davis Adkisson; James A Martin; Richard L Amendola; Curt Milliman; Kelsey A Mauch; Arbindra B Katwal; Mitchell Seyedin; Annuziato Amendola; Philip R Streeter; Joseph A Buckwalter Journal: Am J Sports Med Date: 2010-04-27 Impact factor: 6.202
Authors: Dexter Seow; Youichi Yasui; Eoghan T Hurley; Andrew W Ross; Christopher D Murawski; Yoshiharu Shimozono; John G Kennedy Journal: Am J Sports Med Date: 2017-08-11 Impact factor: 6.202
Authors: A Marmotti; M Bruzzone; D E Bonasia; F Castoldi; R Rossi; L Piras; A Maiello; C Realmuto; G M Peretti Journal: Knee Surg Sports Traumatol Arthrosc Date: 2012-02-21 Impact factor: 4.342
Authors: A Marmotti; D E Bonasia; M Bruzzone; R Rossi; F Castoldi; G Collo; C Realmuto; C Tarella; G M Peretti Journal: Knee Surg Sports Traumatol Arthrosc Date: 2012-11-10 Impact factor: 4.342
Authors: Piera Smeriglio; Janice H Lai; Lakshmi Dhulipala; Anthony W Behn; Stuart B Goodman; Robert L Smith; William J Maloney; Fan Yang; Nidhi Bhutani Journal: Tissue Eng Part A Date: 2014-10-01 Impact factor: 3.845