| Literature DB >> 34627948 |
Elizabeth A Odegard1, Heidi L Meeds1, Steven B Kleiboeker2, Assem Ziady3, Anthony Sabulski4, Sonata Jodele4, Alix E Seif5, Stella M Davies4, Benjamin L Laskin6, Jason T Blackard7.
Abstract
BK polyomavirus (BKPyV) is a ubiquitous pathogen that typically results in asymptomatic infection. However, in immunocompromised individuals, BKPyV viral shedding in the urine can reach 109 copies per mL. These high viral levels within urine provide ideal samples for next-generation sequencing to accurately determine BKPyV genotype and identify mutations associated with pathogenesis. Sequencing data obtained can be further analyzed to better understand and characterize the genetic diversity present in BKPyV. Here, methods are described for the successful extraction of viral DNA from urine and the subsequent amplification methods to prepare a sample for next-generation sequencing.Entities:
Keywords: BK polyomavirus (BKPyV); Diversity; Genotype; Rolling circle amplification; Variation
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Year: 2021 PMID： 34627948 PMCID： PMC8881803 DOI： 10.1016/j.jviromet.2021.114319
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014