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Literature DB >> 34604448

U2.3 Precursor Small Nuclear RNA in vitro Processing Assay.

Chan Lin¹, Yujie Feng¹, Xueyan Peng¹, Jiaming Wu¹, Weili Wang¹, Yunfeng Liu¹.

Abstract

Small nuclear RNAs (snRNAs) are vital for eukaryotic cell activities and play important roles in pre-mRNA splicing. The molecular mechanism underlying the transcription of snRNA, regulated via upstream/downstream cis-elements and relevant trans-elements, has been investigated in detail using cell-free extracts. However, the processing of precursor snRNA (pre-snRNA), which is required by 3' end maturation of pre-snRNA, remains unclear as a proper processing assay is difficult to develop in vitro. Here, we present an in vitro method using synthetic labeled RNA as substrates to study the 3' cleavage of pre-snRNA.

Entities: Chemical

Keywords: Arabidopsis; In vitro; Pre-snRNA; Processing; snRNA

Year: 2021 PMID： 34604448 PMCID： PMC8443454 DOI： 10.21769/BioProtoc.4142

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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References

9 in total

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U2.3 Precursor Small Nuclear RNA in vitro Processing Assay.

Review 1. Small nuclear RNA genes: a model system to study fundamental mechanisms of transcription.

2. 3'-box-dependent processing of human pre-U1 snRNA requires a combination of RNA and protein co-factors.

3. Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II.

4. The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.

5. A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II.

Review 6. snRNA 3' end formation: the dawn of the Integrator complex.

Review 7. Transcriptional regulation of human small nuclear RNA genes.

8. Structure of U2 snRNA genes of Arabidopsis thaliana and their expression in electroporated plant protoplasts.

9. snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis.