Literature DB >> 34595319

Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage.

Dongsheng Jiang1,2, Juliane C De Vries1, Jana Muschhammer1,3, Anca Sindrilaru1, Karin Scharffetter-Kochanek1.   

Abstract

Neutrophils are the most abundant leukocytes in the blood. In the recent decades, their crucial roles in host defense, immune regulation and tissue damage have been studied in a deeper dimension. In this protocol, we described a mouse model of immune complex-mediated vasculitis in the dorsal skin induced by Arthus reaction, and the subsequent analysis of edema, hemorrhage and tissue damage due to neutrophil activation by means of Evans blue area analysis, histology, and immunofluorescence. This protocol could facilitate the investigation of cellular therapy strategy against over-activated neutrophil-mediated tissue damage.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Evans blue; Neutrophil; Tissue damage; Vasculitis

Year:  2017        PMID： 34595319      PMCID： PMC8438367          DOI： 10.21769/BioProtoc.2660

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  8 in total

1.  Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry.

Authors:  Kirstie Canene-Adams
Journal:  Methods Enzymol       Date:  2013       Impact factor: 1.600

2.  Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing.

Authors:  R N Incani; D J McLaren
Journal:  Parasite Immunol       Date:  1981       Impact factor: 2.280

3.  Site of blood vessel damage and relevance of CD18 in a murine model of immune complex-mediated vasculitis.

Authors:  Anca Sindrilaru; Stephan Seeliger; Jan M Ehrchen; Thorsten Peters; Johannes Roth; Karin Scharffetter-Kochanek; Cord H Sunderkötter
Journal:  J Invest Dermatol       Date:  2006-09-28       Impact factor: 8.551

4.  Neutrophil-mediated cellular cytotoxicity triggered by immobilized aggregated IgG: an in vitro model of cell injury during immune complex diseases.

Authors:  F Dallegri; F Patrone; G Frumento; A Ballestrero; C Sacchetti
Journal:  J Clin Immunol       Date:  1984-11       Impact factor: 8.317

5.  α-1-Antitrypsin and IFN-γ reduce the severity of IC-mediated vasculitis by regulation of leukocyte recruitment in vivo.

Authors:  Micha Feld; Tobias Goerge; Carina Hillgruber; Annika Kathrin Steingräber; Michaela Fastrich; Victoria Shpacovitch; Martin Steinhoff
Journal:  J Invest Dermatol       Date:  2012-05-10       Impact factor: 8.551

6.  Inflammation induces hemorrhage in thrombocytopenia.

Authors:  Tobias Goerge; Benoit Ho-Tin-Noe; Carla Carbo; Charaf Benarafa; Eileen Remold-O'Donnell; Bing-Qiao Zhao; Stephen M Cifuni; Denisa D Wagner
Journal:  Blood       Date:  2008-02-06       Impact factor: 22.113

7.  Neutrophil extracellular traps directly induce epithelial and endothelial cell death: a predominant role of histones.

Authors:  Mona Saffarzadeh; Christiane Juenemann; Markus A Queisser; Guenter Lochnit; Guillermo Barreto; Sebastian P Galuska; Juergen Lohmeyer; Klaus T Preissner
Journal:  PLoS One       Date:  2012-02-28       Impact factor: 3.240

8.  Suppression of Neutrophil-Mediated Tissue Damage-A Novel Skill of Mesenchymal Stem Cells.

Authors:  Dongsheng Jiang; Jana Muschhammer; Yu Qi; Andrea Kügler; Juliane C de Vries; Mona Saffarzadeh; Anca Sindrilaru; Seppe Vander Beken; Meinhard Wlaschek; Mark A Kluth; Christoph Ganss; Natasha Y Frank; Markus H Frank; Klaus T Preissner; Karin Scharffetter-Kochanek
Journal:  Stem Cells       Date:  2016-06-27       Impact factor: 6.277
  8 in total

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