Literature DB >> 24182927

Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry.

Kirstie Canene-Adams1.   

Abstract

The purpose of this protocol is to take any biopsy or whole organ tissue from animals or human, formalin-fix the specimen to preserve the current state of the tissue, and embed it into a paraffin block and for future immunohistochemistry experiments (If you intend to fix cells, check the alternative protocols: Preparation of Cells for Microscopy using Cytospin, Preparation of Cells for Microscopy using Chamber Slides and Coverslips, or Preparation of Cells for Microscopy using 'Cell Blocks').
© 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Fixation of tissue; Formalin-fixed paraffin-embedded tissue (FFPET); Immunohistochemistry; Tissue process and embedded; Tissue section

Mesh：

Substances：

Year:  2013        PMID： 24182927     DOI： 10.1016/B978-0-12-420067-8.00015-5

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  29 in total

1.  DNA damage measurements within tissue samples with Repair Assisted Damage Detection (RADD).

Authors:  Kevin J Lee; Elise Mann; Luciana Madeira da Silva; Jennifer Scalici; Natalie R Gassman
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2.  Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells.

Authors:  Yueying Li; Yong Hai; Jiayu Chen; Tie Liu
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3.  The Relationship between KIT Copy Number Variation, Protein Expression, and Angiogenesis in Sporadic Breast Cancer.

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4.  IRF4 deficiency vulnerates B-cell progeny for leukemogenesis via somatically acquired Jak3 mutations conferring IL-7 hypersensitivity.

Authors:  Dennis Das Gupta; Christoph Paul; Nadine Samel; Maria Bieringer; Daniel Staudenraus; Federico Marini; Hartmann Raifer; Lisa Menke; Lea Hansal; Bärbel Camara; Edith Roth; Patrick Daum; Michael Wanzel; Marco Mernberger; Andrea Nist; Uta-Maria Bauer; Frederik Helmprobst; Malte Buchholz; Katrin Roth; Lorenz Bastian; Alina M Hartmann; Claudia Baldus; Koichi Ikuta; Andreas Neubauer; Andreas Burchert; Hans-Martin Jäck; Matthias Klein; Tobias Bopp; Thorsten Stiewe; Axel Pagenstecher; Michael Lohoff
Journal:  Cell Death Differ       Date:  2022-04-22       Impact factor: 15.828

5.  Investigation of Inter- and Intratumoral Heterogeneity of Glioblastoma Using TOF-SIMS.

Authors:  Samvel K Gularyan; Alexander A Gulin; Ksenia S Anufrieva; Victoria O Shender; Michail I Shakhparonov; Soniya Bastola; Nadezhda V Antipova; Tatiana F Kovalenko; Yury P Rubtsov; Yaroslav A Latyshev; Alexander A Potapov; Marat S Pavlyukov
Journal:  Mol Cell Proteomics       Date:  2020-04-06       Impact factor: 5.911

6.  Preparation of Three-dimensional (3-D) Human Liver (HepaRG) Cultures for Histochemical and Immunohistochemical Staining and Light Microscopic Evaluation.

Authors:  Natasha P Clayton; Alanna Burwell; Heather Jensen; Barbara F Williams; Quashana D Brown; Pamela Ovwigho; Sreenivasa Ramaiahgari; Tonia Hermon; Darlene Dixon
Journal:  Toxicol Pathol       Date:  2018-08-08       Impact factor: 1.902

7.  Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species.

Authors:  Catherine E Vrentas; Paola M Boggiatto; Robert G Schaut; Steven C Olsen
Journal:  J Vis Exp       Date:  2018-05-19       Impact factor: 1.355

8.  TRF2 Overexpression at the Surgical Resection Margin: A Potential Predictive Biomarker in Oral Squamous Cell Carcinoma for Recurrence.

Authors:  Madhabananda Kar; Mahesh Sultania; Souvick Roy; Swatishree Padhi; Birendranath Banerjee
Journal:  Indian J Surg Oncol       Date:  2020-02-19

9.  Characterization of the Sheep Round Window Membrane.

Authors:  S Han; H Suzuki-Kerr; M Suwantika; R S Telang; D A Gerneke; P V Anekal; P Bird; S M Vlajkovic; P R Thorne
Journal:  J Assoc Res Otolaryngol       Date:  2020-11-30

10.  Single acquisition label-free histology-like imaging with dual-contrast photoacoustic remote sensing microscopy.

Authors:  Benjamin Ecclestone; Deepak Dinakaran; Parsin Haji Reza
Journal:  J Biomed Opt       Date:  2021-05       Impact factor: 3.170
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