Literature DB >> 34595288

Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning.

Morten Egevang Jørgensen1, Nikolai Wulff2,3, Majse Nafisi3,4, Deyang Xu2,3, Cuiwei Wang2,3, Sophie Konstanze Lambertz2,3, Zeinu Mussa Belew2,3, Hussam Hassan Nour-Eldin2,3.   

Abstract

This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from any synthesizing company. The protocol drastically reduces time and handling between receiving the synthesized DNA fragments and transforming with vector and DNA fragment(s).
Copyright © The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  USERTM cloning ; Cloning of synthesized non-clonal DNA fragments; Fusion of DNA fragments; Geneart; Uracil excision based cloning; uNCDFs

Year:  2017        PMID： 34595288      PMCID： PMC8438380          DOI： 10.21769/BioProtoc.2615

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories.

Authors:  Hussam H Nour-Eldin; Fernando Geu-Flores; Barbara A Halkier
Journal:  Methods Mol Biol       Date:  2010

2.  Software-supported USER cloning strategies for site-directed mutagenesis and DNA assembly.

Authors:  Hans Jasper Genee; Mads Tvillinggaard Bonde; Frederik Otzen Bagger; Jakob Berg Jespersen; Morten O A Sommer; Rasmus Wernersson; Lars Rønn Olsen
Journal:  ACS Synth Biol       Date:  2014-05-21       Impact factor: 5.110

3.  Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning.

Authors:  Ana Mafalda Cavaleiro; Se Hyeuk Kim; Susanna Seppälä; Morten T Nielsen; Morten H H Nørholm
Journal:  ACS Synth Biol       Date:  2015-08-26       Impact factor: 5.110

4.  USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products.

Authors:  Fernando Geu-Flores; Hussam H Nour-Eldin; Morten T Nielsen; Barbara A Halkier
Journal:  Nucleic Acids Res       Date:  2007-03-27       Impact factor: 16.971

5.  Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments.

Authors:  Hussam H Nour-Eldin; Bjarne G Hansen; Morten H H Nørholm; Jacob K Jensen; Barbara A Halkier
Journal:  Nucleic Acids Res       Date:  2006-09-25       Impact factor: 16.971

6.  Origin and evolution of transporter substrate specificity within the NPF family.

Authors:  Morten Egevang Jørgensen; Deyang Xu; Christoph Crocoll; Heidi Asschenfeldt Ernst; David Ramírez; Mohammed Saddik Motawia; Carl Erik Olsen; Osman Mirza; Hussam Hassan Nour-Eldin; Barbara Ann Halkier
Journal:  Elife       Date:  2017-03-03       Impact factor: 8.140
  6 in total

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