| Literature DB >> 20552452 |
Hussam H Nour-Eldin1, Fernando Geu-Flores, Barbara A Halkier.
Abstract
The explosive development of the field of molecular biology has led to the need for simpler and more efficient cloning techniques. These requirements are elegantly met by the ligation-free cloning technique called USER cloning. USER cloning is suitable not only for everyday and high-throughput cloning but also for the one-step construction of complex DNA constructs, which can be achieved in a variant called USER fusion. In this chapter, we present a general protocol for converting any vector into a USER-compatible vector, together with protocols for both USER cloning and USER fusion.Mesh：
Substances：
Year: 2010 PMID： 20552452 DOI： 10.1007/978-1-60761-723-5_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745