Literature DB >> 34595252

Analysis of Xyloglucan Composition in Arabidopsis Leaves.

Javier Sampedro1, Cristina Gianzo1, Esteban Guitián2, Gloria Revilla1, Ignacio Zarra1.   

Abstract

Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species Arabidopsis thaliana. It is based on digestion with an endoglucanase that attacks unsubstituted glucose residues in the backbone. The identities and relative amounts of released xyloglucan fragments are then determined using MALDI-TOF mass spectrometry.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Arabidopsis; Cell wall; Hemicellulose; MALDI-TOF; Primary wall; Xyloglucan

Year:  2017        PMID： 34595252      PMCID： PMC8438494          DOI： 10.21769/BioProtoc.2569

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

1.  Molecular domains of the cellulose/xyloglucan network in the cell walls of higher plants.

Authors:  M Pauly; P Albersheim; A Darvill; W S York
Journal:  Plant J       Date:  1999-12       Impact factor: 6.417

2.  Rapid structural phenotyping of plant cell wall mutants by enzymatic oligosaccharide fingerprinting.

Authors:  Olivier Lerouxel; Tze Siang Choo; Martial Séveno; Björn Usadel; Loïc Faye; Patrice Lerouge; Markus Pauly
Journal:  Plant Physiol       Date:  2002-12       Impact factor: 8.340

Review 3.  Xyloglucan and its interactions with other components of the growing cell wall.

Authors:  Yong Bum Park; Daniel J Cosgrove
Journal:  Plant Cell Physiol       Date:  2015-01-21       Impact factor: 4.927

4.  AtBGAL10 is the main xyloglucan β-galactosidase in Arabidopsis, and its absence results in unusual xyloglucan subunits and growth defects.

Authors:  Javier Sampedro; Cristina Gianzo; Natalia Iglesias; Esteban Guitián; Gloria Revilla; Ignacio Zarra
Journal:  Plant Physiol       Date:  2012-01-20       Impact factor: 8.340

5.  AXY8 encodes an α-fucosidase, underscoring the importance of apoplastic metabolism on the fine structure of Arabidopsis cell wall polysaccharides.

Authors:  Markus Günl; Lutz Neumetzler; Florian Kraemer; Amancio de Souza; Alex Schultink; Maria Pena; William S York; Markus Pauly
Journal:  Plant Cell       Date:  2011-11-11       Impact factor: 11.277

6.  Lack of α-xylosidase activity in Arabidopsis alters xyloglucan composition and results in growth defects.

Authors:  Javier Sampedro; Brenda Pardo; Cristina Gianzo; Esteban Guitián; Gloria Revilla; Ignacio Zarra
Journal:  Plant Physiol       Date:  2010-08-26       Impact factor: 8.340

7.  Generation and structural validation of a library of diverse xyloglucan-derived oligosaccharides, including an update on xyloglucan nomenclature.

Authors:  Sami T Tuomivaara; Katsuro Yaoi; Malcolm A O'Neill; William S York
Journal:  Carbohydr Res       Date:  2014-07-09       Impact factor: 2.104

8.  Soluble and Membrane-Bound β-Glucosidases Are Involved in Trimming the Xyloglucan Backbone.

Authors:  Javier Sampedro; Elene R Valdivia; Patricia Fraga; Natalia Iglesias; Gloria Revilla; Ignacio Zarra
Journal:  Plant Physiol       Date:  2016-12-12       Impact factor: 8.340

9.  AXY3 encodes a α-xylosidase that impacts the structure and accessibility of the hemicellulose xyloglucan in Arabidopsis plant cell walls.

Authors:  Markus Günl; Markus Pauly
Journal:  Planta       Date:  2010-12-18       Impact factor: 4.116

10.  OLIgo mass profiling (OLIMP) of extracellular polysaccharides.

Authors:  Markus Günl; Sascha Gille; Markus Pauly
Journal:  J Vis Exp       Date:  2010-06-20       Impact factor: 1.355
  10 in total

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