Literature DB >> 3459459

DNA-mediated gene transfer into epidermal cells using electroporation.

M Reiss, M M Jastreboff, J R Bertino, R Narayanan.   

Abstract

A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB/c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10(-6) cells per microgram of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.

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Year:  1986        PMID： 3459459     DOI： 10.1016/0006-291x(86)91202-7

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  6 in total

1.  DNA-mediated gene transfection into primary cultures of adult mouse keratinocytes.

Authors:  N A Betz; K J Wolterman; J J Reiners; J C Pelling
Journal:  In Vitro Cell Dev Biol       Date:  1992-03

2.  Differential effects of human papillomavirus type 6, 16, and 18 DNAs on immortalization and transformation of human cervical epithelial cells.

Authors:  G Pecoraro; D Morgan; V Defendi
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

3.  Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.

Authors:  D J Winterbourne; S Thomas; J Hermon-Taylor; I Hussain; A P Johnstone
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

4.  Neoplastic transformation of mouse mammary epithelial cells by deregulated myc expression.

Authors:  N T Telang; M P Osborne; L A Sweterlitsch; R Narayanan
Journal:  Cell Regul       Date:  1990-10

5.  Optimal electric conditions in electrical impulse chemotherapy.

Authors:  M Okino; H Tomie; H Kanesada; M Marumoto; K Esato; H Suzuki
Journal:  Jpn J Cancer Res       Date:  1992-10

6.  Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice.

Authors:  Masahiro Sato; Emi Inada; Issei Saitoh; Masato Ohtsuka; Shingo Nakamura; Takayuki Sakurai; Satoshi Watanabe
Journal:  Biotechnol J       Date:  2013-09-06       Impact factor: 4.677
  6 in total

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