| Literature DB >> 3458219 |
R B Horsch, H J Klee, S Stachel, S C Winans, E W Nester, S G Rogers, R T Fraley.
Abstract
The leaf disc transformation system provides a simple means to score expression of various T-DNA markers within days of infection by Agrobacterium tumefaciens as well as long-term selection for growth of transformed callus and shoots. In this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of A. tumefaciens. We conclude that virC is not essential when the T-DNA is present on a binary vector. The most likely explanation for this is that the virC gene product is involved in generation of a T-DNA intermediate.Entities:
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Year: 1986 PMID： 3458219 PMCID： PMC323340 DOI： 10.1073/pnas.83.8.2571
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205