Nothing lasts forever: understanding microbial biodegradation of polyfluorinated compounds and perfluorinated alkyl substances.

Poly- and perfluorinated chemicals, including perfluorinated alkyl substances (PFAS), are pervasive in today's society, with a negative impact on human and ecosystem health continually emerging. These chemicals are now subject to strict government regulations, leading to costly environmental remediation efforts. Commercial polyfluorinated compounds have been called 'forever chemicals' due to their strong resistance to biological and chemical degradation. Environmental cleanup by bioremediation is not considered practical currently. Implementation of bioremediation will require uncovering and understanding the rare microbial successes in degrading these compounds. This review discusses the underlying reasons why microbial degradation of heavily fluorinated compounds is rare. Fluorinated and chlorinated compounds are very different with respect to chemistry and microbial physiology. Moreover, the end product of biodegradation, fluoride, is much more toxic than chloride. It is imperative to understand these limitations, and elucidate physiological mechanisms of defluorination, in order to better discover, study, and engineer bacteria that can efficiently degrade polyfluorinated compounds.

Introduction

Greater than 9000 heavily fluorinated chemicals have been synthesized for commercial applications (Hogue, 2021). The term ‘perfluorinated alkyl substances’ (PFAS) has become pervasive, but many environmentally relevant fluorinated compounds contain carbon atoms bonded to elements other than fluorine, and so they are best termed polyfluorinated. This review will cover polyfluorinated compounds, both aliphatic and aromatic (Fig. 1). It will discuss perfluorinated compounds and compounds with perfluorinated alkyl groups bonded to other functional groups. While fluoroacetate is not a polyfluorinated compound, it is discussed here because it is the most well‐studied compound with respect to biodefluorination. The knowledge gained from those studies is important to inform ongoing studies with polyfluorinated compounds. The industrial uses of polyfluorinated compounds and moieties have expanded exponentially, being used in more than 200 distinct applications (Glüge et al., 2020). The goal is to present microbial successes and failures in biodegrading polyfluorinated compounds and the underlying reasons for those outcomes. It is important to better understand the chemistry, microbiology and evolutionary history underlying the many failures in order that we may better find and, optimistically, engineer successes.

Fig. 1

Common fluorinated compounds found in the world. The only natural product, fluoroacetate, has a single fluorine substituent. The others are industrial products and are polyfluorinated. Tetrafluoroethene (lower left) is typically not used directly but is polymerized to make the non‐stick polymer Teflon®.

Polyfluorinated compounds are found in every corner of earth, and human and ecosystem toxicity are now well documented (Ahrens and Bundschuh, 2014; Sunderland et al., 2019; Sinclair et al., 2020; Brase et al., 2021; Podder et al., 2021; Rice et al., 2021). Most recently, negative impact of PFAS exposure includes more severe disease progression following COVID‐19 infection (Grandjean et al., 2020). Environmental remediation efforts are increasing exponentially, with billions of dollars being earmarked for the remediation and litigation of polyfluorinated chemicals (Gardella, 2020; Chesler et al., 2021; Cordner et al., 2021; Lim, 2021). However, designating heavily fluorinated compounds as ‘forever’ chemicals has hindered progress on effective remediation strategies. The term signals to the public, regulators and environmental engineers that nature will never destroy them a priori. This review takes the stance that it is premature to preclude bioremediation of polyfluorinated compounds. Biodegradation removes chemicals permanently and is a relatively inexpensive option for eliminating low concentrations of widely distributed pollutants (Alexander, 1999; Wackett and Hershberger, 2001; Parales et al., 2002; Dvořák et al., 2017). In that context, bioremediation is often the method of choice for gasoline spills, groundwater plumes of chlorinated solvents and general industrial waste captured in holding ponds.

Fluorinated compounds pose a new challenge to microbial metabolism and hence to the use of microbes for bioremediation. There is a need for more fundamental research on how microbes interact and react with organic and inorganic fluorine. Fluorine differs from the other halogen elements and chemical functional groups in many of its properties (Aigueperse et al., 2000; Clark, 2002; Jeschke, 2004; Kirsch, 2004; Emsley, 2011). This review will highlight the uniqueness of organofluorine chemistry and consider microbial metabolism in light of that. Overall, the microbial metabolic degradation of heavily fluorinated compounds is a rare phenotype, but one that must be studied at a fundamental level in order to identify, and hopefully engineer, microbes for more rapid biodegradation. Those doing enrichments or molecular engineering will equally benefit from a background understanding of fluorine chemical reactivity, interaction of organofluorine with biomolecules, a natural history of fluorinated compounds in the environment and the potential toxicity of metabolic products of defluorination metabolism. This contribution will review the published literature on fluorine chemistry and biology with the goal of stimulating efforts to better understand and implement PFAS biodegradation.

Polyfluorinated, including perfluorinated, compounds are biodegradable

While news reports continue to describe polyfluorinated compounds as ‘forever’ chemicals, the scientific literature details the biodegradation of multiple fluorinated chemicals, including PFAS. Specifically, two recent papers in Environmental Science and Technology describe a single bacterium and a consortium, respectively, that catalyze defluorination of PFAS (Huang and Jaffé, 2019; Yu et al., 2020). In Huang and Jaffé (2019), microbial incubations with perfluooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) led to their disappearance concurrent with fluoride anion release, and proper controls were performed to rule out artifacts. Both studies identified defluorinated products derived from the parent compound. Huang and Jaffé showed defluorination concomitant with a progressive shortening of the molecule, with perfluorooctanoate proceeding to perfluoroheptanoate and to perfluorohexanoate and so on (Huang and Jaffé, 2019). A similar degradative series has been demonstrated in chemical studies using strong oxidants or reductants, with proposed radical intermediates causing decarboxylation and subsequent defluorination (Singh et al., 2019; Su et al., 2019; Filho and Souza, 2020; Liu et al., 2021; Palma et al., 2021). Given that radical decarboxylation reactions are well known in biological systems (Liu et al., 2018, 2018,2018, 2018; Michlits et al., 2020; Rodrigues et al., 2020; Pastore et al., 2021), a radical‐based mechanism for perfluorooctanoate was proposed (Wackett and Robinson, 2020). Moreover, radical desulfonation enzymology has been described (Peck et al., 2019; Dawson et al., 2021), and radical desulfonation chemistry for PFAS has been described (Niu et al., 2016). Another major class of polyfluorinated compounds are the fluoroteleomers, which typically consists of a polyfluorinated chain bonded to several methylene carbon atoms (Kwiatkowski et al., 2020). In one recent example, 6:2 fluoroteleomer sulfonic acid is biodegraded via attack at the carbon to sulfur bond to produce a fluoroteleomer aldehyde, oxidized to a carboxylic acid, and subsequently defluorinated by HF elimination from the carbon atoms alpha and beta to the carboxylic acid group (Shaw et al., 2019).

Collectively, these studies on the microbiology, enzyme biochemistry and chemistry of polyfluorinated chemicals demonstrate that they are chemically degradable and biodegradable. Indeed, a large industry focusing on remediation has arisen using physical and chemical methods (Batelle, 2021; Cordner et al., 2021; Envirogen Technologies, 2021; Lim, 2021). Incineration has been investigated, but it is energy intensive for low concentrations in water and may produce hazardous hydrogen fluoride in the off gases (Winchell et al., 2021). In addition, electrochemistry, plasma arc technology, and chemical oxidants and reductants are being employed (Singh et al., 2019; Su et al., 2019; Filho and de Souza, 2020; Liu et al., 2021; Palma et al., 2021). However, the major drawback to these methods are their non‐specificity, expense and the chances for producing toxic, reactive compounds as byproducts. In this context, reverse osmosis and nanofiltration have been emerging as important water treatment methods (Mastropietro et al., 2021). Overall, the most common method for water remediation of polyfluorinated compounds is surface adsorption. Activated carbon is often used because of its relative low cost and familiarity to the water treatment communities (Belkouteb et al., 2020; Liu et al., 2020; Sonmez et al., 2021). Carbon filtration is known to remove many organic contaminants, often present at higher concentration than the fluorinated compounds, resulting in competition for binding sites on the adsorbent. For this reason, there have been accelerated efforts to produce more specific adsorbents, and new companies have emerged from these technologies (Ching et al., 2020; Maga et al., 2021).

Biodegradation of fluorinated compounds, as observed to date, occurs on scales of weeks and months, the range of chemicals degraded is limited, and there are few enzymes yet identified that represent the different chemical mechanisms of defluorination (Bondar et al., 1998; Hasan et al., 2011; Tiedt et al., 2016; Huang and Jaffé, 2019; Yu et al., 2020). A rare example is the enzyme fluoroacetate dehalogenase, which has been studied structurally and mechanistically in significant detail (Goldman, 1965; Kurihara et al., 2003; Donnelly and Murphy, 2009; Chan et al., 2011; Chan et al., 2011; Nakayama et al., 2012)). However, the reaction is likely not representative of how polyfluorinated compounds are biodegraded. Difluoroacetate has been found to be much more recalcitrant to biodegradation (Alexandrino et al., 2018), while dichloroacetate is readily biodegraded (Slater et al., 1985; Busto et al., 1992; Thomas et al., 1992; Blackburn et al., 2000; Dixon et al., 2000; Pandey et al., 2017; Chen et al., 2021). Recently, the enzymatic defluorination of difluoracetate and 2,3,3,3‐tetrafluoropropionic acid has been reported (Li et al., 2019; Yue et al., 2021). While the lesser fluorinated analogs are not major pollution problems, trifluoroacetate is used in larger scale industrially, and bioremediation systems have been sought, such as in peptide synthesis companies where this strong acid is used in deblocking chemistry (Sakakibara and Inukai, 1965; Pearson et al., 1989). Interestingly, the biodegradation of trifluoroacetate was initially reported in 1994 (Visscher et al., 1994). However, that enrichment has not been made available, and all efforts to reproduce that result have been unsuccessful till date (Ochoa‐Herrera et al., 2016; Alexandrino et al., 2018; Yu et al., 2020). The report by Huang and Jaffé showed the formation and disappearance of perfluorobutyrate as an intermediate (Huang and Jaffé, 2019), but biodegradation of trifluoacetate was not examined in that study.

In total, the evidence that fluorinated compounds, including polyfluorinated compounds, are biodegraded is compelling. However, it is also evident that this metabolism is confined to a small number of microbial cultures. In addition, when it does occur, the rates of defluorination of heavily fluorinated compounds is low. The probable reasons for the rarity and modest rates of biodegradation are discussed below.

In biology, fluorine is very rare while chlorine, bromine and iodide are much more prevalent

The halogen elements on earth are largely combined with other elements and in that largely inorganic form, fluorine is the most abundant of the halogens on Earth and is overall the 13th most abundant element (Allegre et al., 1995; McDonough, 2021). Despite that, fluorine is relatively insignificant biologically, and this contrasts with the halogens chlorine, bromine and iodine. Cellular chloride transport is an important physiological function in bacteria, plants and mammals (Chen, 2005). The genomes of many bacteria and archaea encode a Clc‐type chloride channel to import and maintain chloride balance (Iyer et al., 2002). In Escherichia coli, a mutation in the chloride channel showed it to be essential for pH homeostasis and acid stress survival (Maduke et al., 1999). Thousands of chlorinated natural products have been identified over the last few decades (Fig. 2) (Gribble, 2012, 2015; Dong et al., 2020; Ludewig et al., 2020), and brominated natural products are common in marine environments (Küpper and Carrano, 2019). Certain algae accumulate high levels of iodine (Mondal et al., 2021), and iodine represents more than half the mass of the human hormone thyroxine (Thomas et al., 2009). Indeed, many chlorinated, brominated and iodinated natural products contain four or more halogen substituents (Fig. 2). Multiple enzymes have been identified that produce organohalides, largely containing chlorine, bromine or iodine. In fact, six different classes of halogenase enzymes are known (Walker and Chang, 2014; Agarwal et al., 2017; Murphy et al., 2017; Fejzagić et al., 2019; Liu et al., 2021). Halogenase activity has apparently evolved independently from different protein folds and utilizing different (or no) cofactors. The classes are as follows: (1) heme‐iron haloperoxidases, (2) non‐heme iron halogenases, (3) flavin halogenases, (4) S‐adenosyl‐l‐methionine halogenases, (5) vanadium haloperoxidases and (6) cofactor‐free haloperoxidases.

Fig. 2

Comparing and contrasting (A) fluorinated natural products (NPs) and (B) chlorinated, brominated and iodinated NPs.

A. Fluorinated NPs have only a single fluorine substituent and are typically analogs of biomolecules: I is 18‐fluorosteric acid, II is fluoroacetone, III is fluorocitric acid, IV is fluorothreonine, V is nucleocidin.

B. NPs Cl, Br, and I may contain multiple halogen substituents, examples with 4–6 per molecule are shown. VI, VII, and IX are marine natural products. VIII is sigillin A, a deterrent produced by a flea. X is the human hormone thyroxine.

By contrast, bacteria and other organisms do not employ fluorine as they do chlorine and other halogens. Fluoride is not accumulated. In fact, it is highly toxic to bacteria, as discussed in a later section. Moreover, biosynthesis of organofluorides by all living things is rare (Murphy et al., 2003; Fincker and Spormann, 2017). The naturally occurring organofluorine compounds are singly fluorinated (Fig. 2) and are typically analogs of common metabolites, such as acetate, citrate, amino acids and nucleotides. This contrasts with chlorinated and brominated natural products, which are often unique structures and may contain a large number of halogen substituents.

It is hypothesized here that the paucity of natural fluorinated compounds compared to other organohalides is one factor underlying the rarity of PFAS biodegradation. It is well accepted that dehalogenases reactive with organochlorine and organobromine compounds have an ancient evolutionary origin and processed natural products prior to industrialization (Leisinger et al., 1994; Valverde et al., 2004; Quack et al., 2007; Futagami et al., 2008; Liang et al., 2012; Hug et al., 2013; Richardson, 2013; Jugder et al., 2016). Some aromatic natural products substituted with chlorine and bromine substituents (Fig. 2) resemble specific industrial chlorinated biphenyl and brominated aromatic ethers (Hou et al., 2021). Polyhalogenated methanes are also natural products (Gribble, 2015) and may have primed the biodegradation of industrial halomethane solvents. For example, a bacterium isolated from a bioreactor degrading dichloromethane contains a dehalogenase that produces formaldehyde, which feeds into the organism’s C1 metabolic pathways. The enzyme dichloromethane dehalogenase is found broadly with widely divergent sequences, consistent with a long evolutionary trajectory. Moreover, it may have evolved under selection for metabolizing the natural product dibromomethane (Quack et al., 2007). The enzyme has a higher k cat and a lower KM for dibromomethane than dichloromethane (Scholtz et al., 1998). In contrast, there is evidence for newly evolved dehalogenases that act on anthropogenic organochloride pesticides (Wackett, 2004; Copley, 2009). Two example enzymes for which there is good evidence for recent evolution are atrazine chlorohydrolase (Seffernick and Wackett, 2001) and 1,3‐dichloropropene dehalogenase (Poelarends and Whitman, 2004).

Fluorine‐containing organic compounds are completely different. There are on the order of a dozen fluorine‐containing natural products and well over one million synthetic organic compounds containing fluorine, as found in a search of PubChem (Kim et al., 2021). The synthetic compounds have only entered commerce, and the environment, in the last few decades. These new organofluorine compounds have been thrust upon relatively unprepared microbial populations. This contrasts with the earlier explosion in synthesis of organochloride commercial chemicals in the 1930s and 1940s that met a microbial population exposed to highly chlorinated natural products for millions of years (Fig. 2). Clearly, microbes are at an evolutionary disadvantage with respect to PFAS and other fluorinated organic chemicals.

Fluorine chemistry is different from other halogens, fluorine microbiology must differ too

Overlaid on this evolutionary consideration are the chemical impediments to the biological disposition of organofluorides, which will be discussed here. However, only a relatively cursory treatment of organofluorine chemistry will be presented, and the reader is recommended to consult more detailed and excellent reviews published elsewhere (Kissa, 2001; Smart, 2001; Dolbier, 2005; O'Hagan, 2008; Hügel and Jackson, 2012).

In the microbiological literature, the rarity of organofluorine degradation is often attributed to the C–F bond being chemically much harder to cleave than other C‐halogen bonds in comparable compounds. This is an oversimplification. Indeed, it is not universally correct because in nucleophilic aromatic substitution reactions, the C–F bond is more readily cleaved than comparable C–Cl and C–Br bonds. That knowledge underlies the use of 1‐fluoro‐2,4‐nitrobenzene, or Sanger’s reagent, for reaction with, and development of a detection method for, amino acids (Sanger, 1945). In addition, as previously discussed, many bacteria produce a fluoroacetate dehalogenase that hydrolyzes a natural product in which the single C–F bond is reasonably reactive, activated by the adjacent carboxylate moiety. This review article seeks to describe the features of C–F chemistry that most relate to the variable biodegradability of different organofluorines as it pertains to their structures and individual reactivities.

While it is difficult to completely generalize, some chemical properties of fluorine and organofluorine compounds can be described (Table 1). Fluorine is the most electronegative atom, which is why organofluoride degradation invariably leads to the electrons leaving with the fluorine atom as an anion. The small size of fluorine is important in the use of fluorine in drug development, since fluorine is not too much larger than a hydrogen substituent and can often substitute sterically without compromising target binding. Such a substitution can serve to substantially moderate drug clearance, an important consideration in drug efficacy (Meanwell, 2018; Al‐Harthy et al., 2020; Hevey, 2021; Richardson, 2021). The lower reactivity of designed organofluorine drugs with Phase I drug metabolism enzymes, typically oxygenases, is paralleled by a typically lesser biodegradation by bacterial oxygenases that act on hydrocarbon substrates in the environment. This has resulted in a recent trend of an increased incorporation of fluorine into agrichemicals to increase environmental lifetime and efficacy (Jeschke, 2017; Burriss et al., 2018; Ogawa et al., 2020). Additionally, fluoride anions interact much more strongly with water compared to other halide anions and this might partially explain why biological systems have rarely incorporated fluoride into biomolecules (Smart, 2001; Tahaikt et al., 2007). This also relates to fluoride toxicity to cells, which will be discussed later.

Table 1

Chemical properties of fluorine relevant to microbial physiology.

General

Highest electronegativity by far

Smaller than any carbon substituent after hydrogen

Fluoride ion is highly solvated in water

Forms tight bonds with carbon, wide variability in bond strength

Low polarizability

In partially fluorinated molecules

Overall molecule often very polar

Variable reactivity

In perfluorocarbons (compounds with only C–C and C–F bonds)

Often lower boiling than the analogous hydrocarbons of much lower MW

High vapor pressures

Poorly soluble in water and organic solvents

Poorly reactive with many reagents

The carbon‐to‐fluorine bond strength is often reported as the strongest known bond, but the variation in bond strength is considerable (Table 1). For example, the bond strength for CF3–F is 130.5 kcal/mol whereas CH3–F is 108.3 kcal/mol. Another literature source reports a C–F bond strength of 154 kcal/mol in hexafluorobenzene (Edelbach and Jones, 1997). Yet, as previously discussed, molecules such as fluoroacetate (Goldman, 1965; Nakayama et al., 2012) and various fluoronitrobenzenes (Zhao et al., 2014; Xu et al., 2019) are relatively reactive, or can be activated, and are readily biodegraded by microbes, so the C–F bond is not uniformly recalcitrant to microbial disruption. Moreover, bond strength is not the sole determinant of reactivity in organofluorine chemical reactions. For example, while C–F bonds are generally stronger than C–H bonds in aromatic and aliphatic compounds containing both, chemists have learned to selectively cleave either bond using metallo‐catalysts (Eisenstein et al., 2017). Selective C–F cleavage takes advantage of bond cleavage mechanisms in which the more exothermic fluoride forming reaction is favored. In general, aryl and olefinic fluorine compounds are more reactive than perfluoroalkanes since the π‐bonds are subject to nucleophilic attack and fluoride is a good leaving group in metal‐catalyzed reactions of this type (Kiplinger et al., 1994). Defluorination of fluoroolefins with replacement by carbon dioxide was shown to be carried out in good yield by copper catalysts (Gao et al., 2020). Recently, a perfluoroalkenoic acid was shown to undergo defluorination at an olefinic carbon by a microbial consortium (Yu et al., 2020).

Perfluoroalkanes are generally less reactive, but are not completely inert and are capable of undergoing reductive defluorination (Saunders, 1996). However, reduction of non‐branched fluorocarbons containing only secondary and primary C‐F bonds is rare. This is due to the low reduction potentials, generally less than −2.7 volts (V) (Park et al., 2009).

Fortunately, most commercially important PFAS are not perfluoroalkanes, but contain other functional groups. Two prominent examples are perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS) and derivatives thereof (Wang et al., 2019). In addition to C–F bonds, these molecules contain C–C(carboxylate) and C–S(sulfonate) bonds that represent another weaker point of attack. Indeed, one‐electron reduction or oxidation of these bonds to generate a carbon‐centered radical, with subsequent installation of a hydroxyl group, is plausible (Fig. 3). This will generate a shorter chain by one via facile gem‐elimination and hydrolysis reactions, and that cycle will repeat (Fig. 3). That is a plausible biodegradation mechanism, and there is precedent for this with non‐biological oxidative and reductive chemistry as has been demonstrated in numerous publications (Park et al., 2009; Liu et al., 2021; Palma et al., 2021).

Fig. 3

A proposed mechanism for the complete defluorination of perfluorooctanoate. The mechanism shows initiating cleavage of the C1‐C2 bond in a radical fashion and quenching of the radical to make a C7 pefluorinated alcohol. The reactions of the alcohol to the carboxylic acid on the right are non‐enzymatic and rapid. The resultant C7 carboxylic acid can undergo the same reaction cycle to make a C6 carboxylate, and then shorter chains, iteratively.

Another distinctive chemical feature of fluorine in organic molecules has relevance to the availability and binding of these chemicals in biological systems. Perfluorocarbons have very low water solubility (Dalvi and Rossky, 2010). In fact, perfluorocarbons are poorly soluble in organic solvents and thus can form a third ‘fluorous phase’ when added to a two‐phase water/organic solvent mixture (Studer et al., 1997; Dobbs and Kimberley, 2002). Hydrofluorocarbons are much more water soluble and perfluoro carboxylate and sulfonate compounds show greatly variable solubility depending upon their carbon chain length. With longer chain lengths, water solubility decreases. PFAS compounds in natural waters, where their concentrations are typically parts per billion or parts per trillion, will be dissolved.

Although solubilities can be measured directly, much less is known about how PFAS compounds such as PFOA and PFOS might move out of an aqueous environment and into bacterial cells. There is evidence that these compounds can partition into cellular membranes passively (Fitzgerald et al., 2018b); in one case, this was shown to affect the quorum sensing response (Fitzgerald et al., 2018a). It is less clear if these molecules are actively transported, perhaps adventitiously by transporters evolved for other uptake functions.

Fluorinated molecules, even perfluorinated, can bind to non‐catalytic proteins such as bovine serum albumin (Fedorenko et al., 2021). This indicates that natural proteins and their amino acid side chains have the potential for evolving pre‐equilibrium binding of these molecules as substrates. Indeed, a recent study has analyzed the human protein ‘interactome’ of perfluorooctanephosphonic acid (PFOPA) and identified 469 PFOPA‐binding proteins (Zhang et al., 2021).

Mechanisms and limitations for microbial organofluorine biodegradation

Microbial degradation of organochlorides has been extensively studied, and so it is useful to compare analogous chlorinated and fluorinated compounds here. Studies of microbial dechlorination physiology have revealed five general types of reactions: (1) reductive, (2) oxidative, (3) hydrolytic, (4) substitutive, and (5) eliminative (Fig. 4). Based on known chemistry as previously discussed, the propensity for organofluorine biodegradation following each of these mechanisms will be compared and contrasted with organochlorine biodegradation. Since this review focuses on fluorine, C–F bonds are illustrated. However, it should be understood that there are many more examples of C–Cl bond cleavage for each type of reaction. As previously discussed, fluorine differs from other halogens and so we would anticipate that many dechlorinating enzymes would fail to catalyze defluorination of analogous molecules. Exceptions are known with some reasonably reactive monofluorinated compounds such as fluoroacetate (Kurihara et al., 2000), fluoroatrazine (Seffernick et al., 2000) and 2‐fluorobenzoate (Engesser and Schulte, 1989).

Fig. 4

Generalized mechanisms of defluorination based on examples in the scientific literature. The carbon atoms may be alkyl, olefinic or aromatic. The reactions are simplified to show the key elements of each reaction type. Beta‐elimination can also occur with H‐C‐C‐F containing compounds and produce H+ and F− as products.

Reductive defluorination

In the mid‐twentieth century, many chlorinated synthetic pesticides were considered to be highly persistent in the environment, even non‐biodegradable (Wackett and Robinson, 2020). It was realized in the 1960s that certain of these organochloride and organobromide pesticides underwent reductive dehalogenation in soil (Castro and Belser, 1968). Subsequent research showed that reduced metallo‐enzymes and their cofactors could catalyze reductive dechlorination (Stotter et al., 1977; Schrauzer and Katz, 1978). Subsequently, chloro‐aromatics (Suflita et al., 1982), chloro‐alkenes (Vogel and McCarty, 1985) and chloroalkanes (Gälli and McCarty, 1989) were shown to undergo microbially catalyzed reductive dechlorination (Fig. 4). Enzymes have been purified, structures determined, and reactions shown to be dependent on reduced low‐potential cobalt‐corrinoid cofactors (Neumann et al., 1996; Kräutler et al., 2003; Payne et al., 2015). To this author’s knowledge, none of these well‐characterized dechlorinating and debrominating enzymes have been shown to catalyze reductive defluorination, but reduced corrinoids have been demonstrated to have defluorination activity with polyfluorinated compounds (Ochoa‐Herrera et al., 2016; Liu et al., 2018, 2018,2018, 2018; Sun et al., 2021).

A key feature of reductive dechlorination came to light with the realization that polychlorinated biphenyls (PCBs) and other aromatic and aliphatic compounds can serve as final electron acceptors in respiratory metabolism (Brown et al., 1987; Mohn and Tiedje, 1990; Schumacher and Holliger, 1996; Susarla et al., 1997; Holliger et al., 1998; Smidt et al., 2000; Duhamel and Edwards, 2007). Microorganisms will often utilize respiratory over fermentative metabolism, when they are capable of both, to capture more energy. In this context, anaerobes use many different electron acceptors in place of oxygen to maximize energy acquisition. Some bacteria are now known to catalyze reduction of C–Cl bonds in a manner that is analogous to the reduction of nitrate or sulfate. Halo‐respiring organisms couple dechlorination to electron transport‐driven ATP generation (Smidt and de Vos, 2004). This process of organochlorine and organobromine respiration is now considered to be reasonably common, evolutionarily ancient, and further adapted in response to the many chlorinated and brominated natural products produced in the environment (Mayer‐Blackwell et al., 2016; Tang et al., 2016). The process is thermodynamically feasible and beneficial because the redox potential of the C–Cl bond in many chloroaromatics and chloroaliphatics is in the range of +250 to +600 mV (Holliger et al., 1998). So many simple metabolic oxidation reactions, for example, the two‐electron oxidation of succinate to fumarate at +32 mV (Thauer et al., 1989), have a lower redox potential and could theoretically couple to chloro‐reduction to input electrons into the respiratory chain and ultimately produce ATP for the organism.

C–F bond reduction is not analogous energetically. As discussed previously under the preceding section, the redox potential of −2700 mV or lower is well below any oxidizable growth substrate for bacteria and out of the range of biological redox carriers. PFAS compounds that are more amenable for reduction, such as PFOS and PFOA, are reported to have redox potentials in the −450 mV range (Park et al., 2009). While this is within the low end of redox catalysts in biology, it is much too low for the reduction reaction to be coupled to a respiratory chain and generate ATP for a bacterium. Oxidizable substrates for microbes typically have redox potentials more positive than −450 mV, and so transfer of electron from those substrates to PFAS reduction would be thermodynamically unfavorable. Therefore, unlike C–Cl bond reduction, C–F bond reduction seems unlikely to be coupled to ATP generation.

In light of this, how might the C–F bonds of polyfluorinated compounds with a low but biologically accessible redox range, in the range of −400 to −700 mV, be amenable to reductive defluorination? By way of calibration, the low potential coenzyme for many cellular reduction reactions, NADH, has a standard redox potential of −320 mV and would not directly couple in these reaction. There are, however, several important processes microorganisms have evolved to meet the need for low‐potential reduction metabolism. One is the evolution of enzymatic machinery for the six‐electron reduction of atmospheric dinitrogen to produce two atoms of ammonia, a process known as nitrogen fixation and catalyzed by the enzyme nitrogenase. The redox potential of the nitrogenase Fe–Mo protein is reported to be approximately −540 mV (Milton and Minteer, 2019). Since nitrogen‐fixing bacteria often grow on oxidizable carbon and energy sources with more positive redox potentials than −540 mV, ATP is required to ramp down redox potentials through a series of electron carriers to the Fe‐Mo protein (Rutledge and Tezcan, 2020). Another example of low potential metabolism that has evolved in certain bacteria is represented by enzymes mediating anaerobic growth on benzene‐ring compounds via an initial reduction reaction (Wischgoll et al., 2005; Löffler et al., 2011). The benzene ring is stabilized by a resonance energy of 36 kcal mol−1 over 1,3,5‐hexatriene, so reduction of the benzene ring, as carried out by organic chemists, employs extremely powerful reducing reagents such as elemental sodium in liquid ammonia (Zimmerman, 2012). The bacterium Geobacter metallireducens meets this demand by biosynthesizing a one megadalton enzyme complex that can attain a redox potential of −622 mV (Huwiler et al., 2019). Like nitrogenase, attainment of a requisite ultra‐low redox potential requires ATP.

Indeed, there is precedence for ATP‐dependent reductive defluorination (Tiedt et al., 2016). Thauera aromatica has been shown to catalyze an ATP‐dependent reaction in which 4‐fluorobenzoyl‐CoA is overall reductively defluorinated to benzoyl‐CoA and HF. The reaction is catalyzed by an enzyme homologous to known benzoyl‐CoA reductases and is proposed to proceed via an aromatic ring reduction followed by HF elimination. This is an example of defluorination of a monofluorinated compound but, as discussed above, may prove relevant to polyfluorinated compounds.

Accrued knowledge of PFAS redox chemistry, microbiological redox biochemistry and direct microbiological data (Tiedt et al., 2016) suggests that C–F bond reduction will require ATP, in contrast to C–Cl bond reduction that can generate ATP. Earlier in this review, evolution was said to favor metabolism of organohalides other than organofluorides because of the prevalence of polychlorinated and polybrominated natural products. Another disadvantage for C–F reduction is that it may be selected against in evolution because it provides no benefit, and may even drain cellular energy. This further explains why the reduction of PFOA and PFOS may be rarely identified and relatively slow, consistent with reports of laboratory persistence (Dinglasan et al., 2004; Parsons et al., 2008; Murphy, 2010), and a limited number of recent successful enrichments that show relatively slow biodegradation rates (Huang and Jaffé, 2019; Yu et al., 2020). It is important to understand the molecular basis of the defluorination reactions to better find natural representatives and to potentially re‐engineer microbial ultra‐low redox systems for PFAS biodegradation. Note that nitrogenase is being re‐engineered in this manner (Milton and Minteer, 2019) and is now demonstrated in the laboratory to generate hydrocarbons (Yang et al., 2011) and other organic products (Seefeldt et al., 2013) in an effort to extend the enzyme’s catalysis beyond its evolved function of dinitrogen reduction.

Oxidative defluorination

There are numerous reports of oxidative defluorination, most of which involve aromatic or other ring systems with single fluorine substituents. Excellent review articles are available covering C‐F bond cleavage with a focus on aromatics (Kiel and Engesser, 2015) and reactions catalyzed by metalloenzymes (Wang and Liu, 2020). Many of the metalloenzymes catalyzing defluorination are oxygenases, oxidases and peroxidases. The mechanisms of monodefluorination have been studied with cytochrome P450 monooxygenases (Harkey et al., 2012), dioxygenases (Li et al., 2020; Renganathan, 1989) and oxidases (Li et al., 2020). Recently, bacterial degradation of monofluorinated alkyl chains via oxygenase‐initiated reactions was reported (Xie et al., 2020). This type of reaction is best represented by the second mechanism from the top depicted in Fig. 4.

Bacterial oxidative biodegradation of polyfluorinated olefins is relatively rare. An exception is the biodegradative defluorination of trifluoroethylene by the soluble methane monooxygenase from Methylosinus trichosporium OB3b (Fox et al., 1990). The major product of the reaction was glyoxylate. The rate of oxidation was only 10% of the rate with trichloroethylene. Chlorotrifluoroethylene was oxidized to oxalate, consistent with complete dehalogenation, but the rate was only 25% of that for trifluoroethylene.

The major focus on this review article is on polyfluorinated compounds, particularly aliphatic compounds with carbon atoms containing multiple fluorine substituents. In drug design, it is widely appreciated that substituting trifluoromethyl for methyl groups will block oxygenases that initiate metabolism and thus slow drug clearance (Hagmann, 2008; Sun et al., 2011). Trifluoromethyl groups on aromatic rings often persist, even as the ring may be oxidized, and the three C‐F bonds can end up on trifluorocetate as a metabolic end‐product (Key et al., 1997; Kiel and Engesser, 2015). Difluoromethyl substituents are much less common in commercial compounds. They have been examined as anti‐metabolites and inhibitors in medical chemistry. A difluoromethyl‐analog of progesterone has been suggested to be hydroxylated at the –CHF2 group due to its effectiveness as an irreversible inhibitor. Inhibition is proposed to result from hydroxylation, gem‐elimination and reaction of the resultant acyl fluoride with the cytochrome P450, thus inhibiting the enzyme (Stevens, 1991).

Hydrolytic defluorination

The prototypic bacterial enzyme catalyzing hydrolytic defluorination is fluoroacetate dehalogenase. As previously discussed, fluoroacetate (Fig. 1) is a natural product and highly toxic, so bacteria may benefit from detoxification and acquisition of carbon and energy from the defluorinated product, glycollate (Goldman, 1965). In this context, fluoroacetate is degraded by bacteria isolated from the rumen of cows, which may periodically ingest one or more of the forty plants known to biosynthesize fluoroacetate (Davis et al., 2012). Fluoroacetate dehalogenase enzymes have also been identified in diverse soil bacteria of the genera Moraxella, Delftia, Burkholderia, Pseudomonas and Rhodopseudomonas (Seong et al., 2019). Many of these enzymes isolated as fluoroacetate dehalogenases have some activity with chloroacetate and bromoacetate. However, fluoroacetate typically shows the highest catalytic efficiency, k cat/K M, with enzymes designated as fluoroacetate dehalogenases.

The nature of the relative catalytic efficiency with fluoroacetate has been most well‐studied with the enzyme denoted FacD from Burkholderia sp. FA1 via studies involving X‐ray crystallography, site‐directed mutagenesis, rapid synchotron crystallography and computational studies (Chan et al., 2011; Miranda Rojas et al., 2018; Schulz et al., 2018). The reaction is known to proceed via nucleophilic attack of an aspartate nucleophile to generate an aspartate ester intermediate that is resolved by attack of water activated by an active site histidine. This is a fairly unremarkable mechanism common to many enzymes with a general α/β hydrolase fold. Rather a major factor in reduction of the activation barrier of C‐F bond cleavage is the specific interaction of the fluorine with the halogen pocket. The halogen pocket is defined by the triad of a tryptophan, histidine and tyrosine within a distance of 3.0 to 3.3 Angstrom from the fluorine that defines a fluorine‐specific pocket in the enzyme–substrate complex. Computational studies have further defined optimum bond angles and plausible energy barriers to facilitate C‐F bond cleavage.

Substitutive defluorination

Rumen bacteria with hydrolytic fluoroacetate dehalogenases are known to protect animals that have ingested fluoroacetate‐containing plant material (Loh et al., 2020). However, fluoroacetate is also detoxified by enzymes that substitute atoms for fluorine other than the oxygen of a water molecule, with the nucelophile represented as X− in Fig. 4. These types of reactions have been most studied in plants and animals that use their own enzymes for detoxification. An enzyme was identified in mammals that catalyzed the substitution of the fluorine atom of fluoroacetate with the sulfur atom of the tripeptide glutathione (Soiefer and Kostyniak, 1983). The enzymes, later known as theta‐glutathione‐S‐transferases, are found in non‐mammalian sources as well, so the ability to detoxify fluoroacetate may be found broadly throughout biological systems (Sheehan et al., 2001). Interestingly, an enzyme of the same class is thought to catalyze defluorination of the difluoromethylene moiety of the anesthetic methoxyflurane (Wang et al., 1986).

Eliminative defluorination

The classic eliminative dehalogenation reaction is that catalyzed by the LinA dehydrohalogenase. LinA transforms the pesticide lindane, or gamma‐hexachlorocyclohexane, to 1,3(R),4(S),5(S),6(R)‐pentachlorocyclohexane via the elimination of H+ and Cl− from adjacent carbon atoms, similar to the beta‐elimination of two halide substituents shown in Fig. 4. The reaction has been studied in some detail (Nagata et al., 2001; Manna et al., 2015). Analogous dehydrofluorination reactions have been shown to occur with enzymes in which synthetic fluorinated substrate analogs have been used as mechanistic probes (O'Hagan and Rzepa, 1997; Gulick et al., 2001). Dehydrofluorination of polyfluoroalkanes via non‐enzymatic catalysis is known (Pedler et al., 1972; Li et al., 1998).

There are examples of fluoride eliminations that occur non‐enzymatically after attack by reductive (Tiedt et al., 2017) or oxygenative (Fox et al., 1990) enzymes, as previously discussed. A somewhat novel alpha‐elimination was observed following reduction of fluorotrichloromethane by a bacterial cytochrome P450 followed by chloride elimination, fluorchlorocarbene formation, and then loss of both remaining halide ions (Fig. 4) (Li and Wackett, 1993). Most recently, a beta‐elimination pathway was proposed for the defluorination and complete biodegradation of 3,3,3‐trifluoropropionic acid by a sludge sample from a wastewater treatment plant (Che et al., 2021).

Defluorination produces fluoride, and fluoride is highly toxic to all living things

It is proposed here that there is another very significant reason why so few microbes have evolved the capabilities to biosynthesize or biodegrade organofluoride compounds. With fluoride being the most electronegative element, the C–F bond is invariably cleaved heterologously to yield fluoride. However, fluoride is highly toxic to prokaryotic and eukaryotic cells.–Fluoride and chloride are very different environmentally and biologically. While fluorine is significant in the earth’s crust, it is largely bound up in minerals. The ratio of chloride to fluoride in seawater is > 7600 to 1 (Greenhalgh and Riley, 1961). Chloride, is also much more prevalent in terrestrial water sources, where it can be found at > 4 M in some saline seas. Halophilic bacteria survive and thrive in 4 M chloride concentrations in these environments (Müller and Oren, 2003). Bacteria growing in lower saline environments also use chloride extensively. For example, the light‐driven chloride pump proteins classified as rhodopsins have been extensively studied (Inoue et al., 2015). Some bacteria have been found to contain ~ 1 M chloride intracellularly (Chen, 2005).

Fluoride is not only much less prevalent in natural waters, but it is also highly toxic to organisms in general. The World Health Organization has recommended a human limit for fluoride in drinking water to be 1.5 mg l−1, or 79 μM (Edmunds and Smedley, 2013). In certain waters, fluoride concentrations as low as 26 μM can adversely affect some invertebrates and fishes (Camargo, 2003). Fluoride is also highly toxic to bacteria. This has been exceptionally well documented in many different types of experiments with diverse bacteria (Bhatnagar and Bhatnagar, 2004; Adamek et al., 2005; Ochoa‐Herrera et al., 2009; Baker et al., 2012; Breaker, 2012; Stockbridge et al., 2012; Ji et al., 2014; Nelson et al., 2015; Liao et al., 2017; Liu et al., 2017; Last et al., 2018; Turman et al., 2018; Zhu et al., 2019; Johnston and Strobel, 2020; Dionizio et al., 2021).

What are the mechanisms of fluoride toxicity? In mammals, fluoride can induce oxidative stress, disrupt redox homeostasis, increase protein carbonyl content, alter gene expression and cause apoptosis (Barbier et al., 2010). In bacteria, cellular disruption is largely attributable to inhibition of essential and non‐essential enzymes (Wiseman, 1970). This has been extensively studied in oral bacteria and is one of the reason for the presence of fluoride in toothpastes (Hamilton, 1990). Fluoride is highly inhibitory to metallo‐enzymes, particularly those using magnesium. The inhibition of specific enzymes can be exceedingly strong, with KI values for some enzymes measured to be as low as 2 μM (Guranowski, 1990). Essential enzymes shown to be inhibited by fluoride include pyrophosphatase (Baykov et al., 2000), proton translocating ATPases (Sutton et al., 1987; Sturr and Marquis, 1990) and enolase (Qin et al., 2006) (Fig. 5). The urease of Klebsiella aerogenes has been shown to be inhibited by fluoride (Todd and Hausinger, 2000).

Fig. 5

Visualizing requirements of a bacterial cell found naturally, or via laboratory engineering, that would be capable of rapid defluorination of PFAS. The need for multiple systems to be present simultaneously is shown. First, the fluorinated compound, denoted C–F, may enter the cell passively based on uptake studies. The mechanism of defluorination in this example is reductive. That may require a cofactor such as cobalamin and a low‐potential ferredoxin reducing system. Both the low‐potential electron generating system and the reductive dehalogenase may require ATP. Fluoride, if remaining in the cell, can be inhibitory to the required ATPase, preventing new ATP generation. Fluoride can also inhibit essential cellular enzymes such as pyrophosphatase and enolase. To protect against fluoride toxicity, the cell must sense fluoride, perhaps using a fluoride riboswitch regulatory system, and synthesize a membrane‐bound fluoride exporter system to maintain a low, steady‐state intracellular fluoride concentration.

These findings over the last four decades raise an important question regarding the biodegradation of polyfluorinated compounds. Reductive defluorination and other reactions requiring complex enzyme machinery will almost invariably occur intracellularly and release fluoride, so this is another impediment to microbes degrading highly fluorinated compounds. The intracellular volume of a typical bacterial cell is less that 10−12 μl (Ingraham et al., 1983). Therefore, if there were 108 bacteria per ml and they released one fluorine atom while degrading 10 μM of a fluorinated compound from their surrounding solution, the initial intracellular fluoride concentration would be 100 mM, a highly toxic level. Many PFAS compounds contain ten or more fluorine atoms, compounding the problem.

Fortunately, some bacteria have evolved protective mechanisms of fluoride resistance (Chouhan et al., 2012; Liao et al., 2015; Mukherjee et al., 2017; Chellaiah et al., 2021), and these systems would need to be very effective to protect the cell while biodegrading even low μM levels of polyfluorinated compounds. Given that fluorinated natural products and defluorinating enzymes are rare, this protection has likely evolved to protect against external fluoride. There are many regions in the world where fluoride exceeds healthy levels for humans, and bacterial enzymes would be impacted at those same levels (Amini et al., 2008). Fluoride uptake in bacteria is impacted by the strong H‐bonding propensity of fluoride in water, which causes it to interact more strongly with protons. That raises the pKa to 3.4, which is much higher than the pKa for other hydrogen halides (O’Hagan, 2008). Under mildly acidic aqueous extracellular conditions, sufficient HF will be present. HF readily partitions into bacterial cells, and free fluoride is released under the more neutral pH of the cytoplasm (Ji et al., 2014). If millimolar concentrations are reached, bacterial enzymes will be severely inhibited, as discussed previously.

Two responses are needed to protect the bacterial cells. First, fluoride needs to be sensed. Second, fluoride detection needs to be followed by responses that mitigate against toxic effects. For the latter, the cell is best served by removing fluoride from the cell as a number of enzymes are reversibly inhibited by fluoride, allowing recovery of activity. On the first point, thousands of fluoride‐sensing riboswitches (Fig. 5) have been discovered in many diverse bacterial strains (Baker et al., 2012; Breaker, 2012). Riboswitches are components of certain mRNAs that bind to ligands and subsequently regulate protein expression from that mRNA. Proteins expressed via fluoride riboswitches include higher copy numbers of enolase, stress genes, DNA repair functions and hypothetical proteins. Ultimately, the cell can be rescued from fluoride by producing one or both of two non‐homologous classes of transport proteins (Stockbridge et al., 2012; Ji et al., 2014; Last et al., 2018). One such type of transport is a passive, gradient‐controlled passage of fluoride via what is known as Fluc‐F− channels. The other class are F−/H+ antiporters that act similarly to multi‐drug resistance transporters and use a proton gradient or ATP hydrolysis to expel fluoride against a gradient (Fig. 5).

The toxicity of fluoride raises a key issue for any newly evolving C–F bond cleavage metabolism. Without rapid export of the fluoride product, the metabolism will be counter‐selected against and not be retained in populations. Therefore, this imposes another constraint on biodegradation. In total, effective biodegradation might require a powerful enzyme(s), ATP‐consumption to drive low‐potential C–F bond cleavage and fluoride sensing and export capabilities (Fig. 5). Interestingly, Acidmicrobium sp. A6, which biodegrades PFAS compounds (Huang and Jaffé, 2019), has a putative fluoride ion transporter gene(s) in its genome (S. Huang & P. Jaffé, personal communication).

Prospects and hope

This review has related that polyfluorinated compounds, including PFAS, are biodegradable, the metabolism has not evolved over eons like that for other organohalides, and the chemistry and biology of fluorine make PFAS biodegradation rare. First, microbes have not been long‐exposed to highly‐fluorinated natural products, requiring newly evolved metabolism. Second, evolution and gene spread is driven by natural selection, and metabolism of polyfluorinated compounds may often lack selective benefit. Many PFAS are at or near the carbon dioxide oxidation level, so they are not oxidizable for energy. Moreover, they cannot serve as a final electron acceptor if the redox potential is low enough to preclude a thermodynamic benefit to reduction. Indeed, C–F bond cleavage of multiply fluorinated compounds may require ATP and thus impose a metabolic burden (Fig. 5). If these metabolic impediments hold, then biodegradation will largely be relegated to metabolic ‘accidents.’ That is, high‐powered metabolic enzymes, designed by nature for other metabolism, will potentially cross‐over and react with some polyfluorinated compounds. Third, multiple systems will likely need to be in place for sustained biodegradation. In this context, biodegradation requirements could include low potential redox transfer proteins, enzymes capable of reacting with C–F bonds, transport into the intracellular space and robust fluoride resistance mechanisms (Fig. 5). The latter likely includes active fluoride export proteins and enzymes that are intrinsically more resistant against fluoride inhibition.

Currently, remediation biotechnology uses commercially available cultures of Dehalococcoides for organochloride cleanup (Kanitkar et al., 2016), and it is important, in concluding this review, to consider if organofluorine compounds might also be microbiologically remediated. The tough questions here focus on PFAS, the most recalcitrant of the organofluorine compounds. For that deliberation, it is necessary to address the following questions: (1) Do PFAS degrading organisms exist? (2) Can the mechanisms of those PFAS degraders be elucidated? (3) Can PFAS degrading bacteria ultimately be harnessed for bioremediation?

For the first point, bacteria have already been demonstrated to biodegrade PFAS (Huang and Jaffé, 2019; Yu et al., 2020). Given that there are estimated to be greater than 1030 prokaryotes on earth (Whitman et al., 1998), the vast majority of which are yet unstudied, there are likely to be more PFAS‐metabolizing bacteria uncovered. For the second point, if microorganisms can be shown to biodegrade PFAS in the laboratory, it is reasonable to expect that the genes and enzymes can be identified and studied in detail. The third question is more difficult to answer. The rates of PFAS degradation reported to date are very low (Huang and Jaffé, 2019; Yu et al., 2020), and, currently, they may not be suitable for bioremediation. This impediment could be overcome with discoveries of new organisms or from laboratory engineered microbes derived from insights into the enzymes and mechanisms. There may be questions as to whether regulatory agencies would allow genetically engineered bacteria to be used in PFAS bioremediation, but that is a political, not a scientific, issue. In conclusion, this author is hopeful that real‐world solutions will be forthcoming.

Conflict of interest

None declared.