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Literature DB >> 34558011

Generating Cerebral Organoids from Human Pluripotent Stem Cells.

Leon Chew¹, Adam Añonuevo¹, Erin Knock².

Abstract

3D brain organoids derived from human pluripotent stem cells (hPSCs) possess the remarkable ability to self-organize and differentiate into tissue resembling the early human fetal brain. Brain organoids provide a powerful tool for studying human brain development and disease in an in vitro system. Here we describe a protocol for the differentiation of hPSCs to human cerebral organoids using a commercially available kit (STEMdiff™ Cerebral Organoid Kit) and discuss methods to scale up the protocol in a high-throughput manner.

Entities: Chemical

Keywords: Brain; In vitro; Neural differentiation; Neurodevelopment; Organoid; Stem cells; Three-dimensional

Mesh：

Year: 2022 PMID： 34558011 DOI： 10.1007/978-1-0716-1783-0_15

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

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Generating Cerebral Organoids from Human Pluripotent Stem Cells.

1. Self-formation of optic cups and storable stratified neural retina from human ESCs.

2. Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals.

3. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex.

4. Self-organizing optic-cup morphogenesis in three-dimensional culture.

5. Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture.

6. Cell diversity and network dynamics in photosensitive human brain organoids.

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Review 8. Organogenesis in a dish: modeling development and disease using organoid technologies.

9. Generation of cerebral organoids from human pluripotent stem cells.

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