Roni Rahav-Koren1,2, Shmuel Inbar3,4, Netanella Miller3,4, Amir Wiser3,4, Yael Yagur3,4, Chen Berkowitz5, Sivan Farladansky-Gershnabel3,4, Adrian Shulman3,4, Arie Berkowitz3,4,6. 1. IVF Unit, Department of Obstetrics and Gynecology, Meir Medical Center, 59 Tchernichovsky St, 4428164, Kfar Saba, Israel. rahav.roni@gmail.com. 2. Sackler Faculty of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel. rahav.roni@gmail.com. 3. IVF Unit, Department of Obstetrics and Gynecology, Meir Medical Center, 59 Tchernichovsky St, 4428164, Kfar Saba, Israel. 4. Sackler Faculty of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel. 5. Faculty of Medicine, Hebrew University of Jerusalem, 9112102, Jerusalem, Israel. 6. Assuta Medical Center, 75654, Rishon Letzion, Israel.
Abstract
PURPOSE: Does thawing cleavage embryos and culturing them for transfer as blastocysts improve pregnancy and perinatal outcomes compared to transferring thawed blastocysts? METHODS: Retrospective, observational cohort study performed at two assisted reproductive technology centers, 2014 to 2020. A total of 450 patients with 463 thawed embryo transfer cycles were divided into 2 groups according to the embryonic developmental stage at cryopreservation and transfer: 231 thawed blastocysts (day 5 group) and 232 thawed cleavage embryos that were cultured for 2 days and transferred as blastocysts (day 3-5 group). The two groups were compared for demographics, routine parameters of IVF treatment, pregnancy rates, and perinatal outcomes. RESULTS: Multivariable logistic regression analysis for ongoing pregnancy and delivery demonstrated that the day 3-5 group had a greater likelihood of achieving ongoing pregnancy and delivery compared to the day 5 group (OR 1.58, 95%CI 1.062-2.361, p = 0.024). Perinatal outcomes were comparable between the three groups. CONCLUSION: Our results support culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts to increase chances of ongoing pregnancy and delivery.
PURPOSE: Does thawing cleavage embryos and culturing them for transfer as blastocysts improve pregnancy and perinatal outcomes compared to transferring thawed blastocysts? METHODS: Retrospective, observational cohort study performed at two assisted reproductive technology centers, 2014 to 2020. A total of 450 patients with 463 thawed embryo transfer cycles were divided into 2 groups according to the embryonic developmental stage at cryopreservation and transfer: 231 thawed blastocysts (day 5 group) and 232 thawed cleavage embryos that were cultured for 2 days and transferred as blastocysts (day 3-5 group). The two groups were compared for demographics, routine parameters of IVF treatment, pregnancy rates, and perinatal outcomes. RESULTS: Multivariable logistic regression analysis for ongoing pregnancy and delivery demonstrated that the day 3-5 group had a greater likelihood of achieving ongoing pregnancy and delivery compared to the day 5 group (OR 1.58, 95%CI 1.062-2.361, p = 0.024). Perinatal outcomes were comparable between the three groups. CONCLUSION: Our results support culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts to increase chances of ongoing pregnancy and delivery.
Authors: W P Martins; C O Nastri; L Rienzi; S Z van der Poel; C Gracia; C Racowsky Journal: Ultrasound Obstet Gynecol Date: 2017-04-10 Impact factor: 7.299
Authors: Ange Wang; Anthony Santistevan; Karen Hunter Cohn; Alan Copperman; John Nulsen; Brad T Miller; Eric Widra; Lynn M Westphal; Piraye Yurttas Beim Journal: Fertil Steril Date: 2017-06-01 Impact factor: 7.329
Authors: Yong Soo Hur; Jeong Hyun Park; Eun Kyung Ryu; Hae Jin Yoon; San Hyun Yoon; Chang Young Hur; Won Don Lee; Jin Ho Lim Journal: Clin Exp Reprod Med Date: 2011-06-30