Literature DB >> 34545490

High-Throughput BN-PAGE for Mitochondrial Respiratory Complexes.

Lilian Vincis Pereira Sanglard1, Catherine Colas des Francs-Small2.   

Abstract

Blue native electrophoresis (BN-PAGE) is a highly resolutive method suited to the study of high molecular weight protein complexes between 100 and >3000 kDa. One of the drawbacks of this method is that it is very time-consuming and requires high quantities of purified organelles. Here we describe a high throughput BN-PAGE method allowing to screen libraries of plants potentially altered in respiratory metabolism.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Blue Native PAGE; Immunoblots; Mitochondria; Respiratory complexes

Mesh：

Year:  2022        PMID： 34545490     DOI： 10.1007/978-1-0716-1653-6_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Histochemical staining and quantification of plant mitochondrial respiratory chain complexes using blue-native polyacrylamide gel electrophoresis.

Authors:  Mohammed Sabar; Janneke Balk; Christopher J Leaver
Journal:  Plant J       Date:  2005-12       Impact factor: 6.417

2.  Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis.

Authors:  Etienne H Meyer; Nicolas L Taylor; A Harvey Millar
Journal:  J Proteome Res       Date:  2008-01-12       Impact factor: 4.466

3.  Insights into the composition and assembly of the membrane arm of plant complex I through analysis of subcomplexes in Arabidopsis mutant lines.

Authors:  Etienne H Meyer; Cory Solheim; Sandra K Tanz; Géraldine Bonnard; A Harvey Millar
Journal:  J Biol Chem       Date:  2011-05-23       Impact factor: 5.157

4.  New insights into the composition, molecular mass and stoichiometry of the protein complexes of plant mitochondria.

Authors:  L Jänsch; V Kruft; U K Schmitz; H P Braun
Journal:  Plant J       Date:  1996-03       Impact factor: 6.417

Review 5.  Proteomic approach to characterize mitochondrial complex I from plants.

Authors:  Jennifer Klodmann; Hans-Peter Braun
Journal:  Phytochemistry       Date:  2010-12-15       Impact factor: 4.072

6.  Defining the protein complex proteome of plant mitochondria.

Authors:  Jennifer Klodmann; Michael Senkler; Christina Rode; Hans-Peter Braun
Journal:  Plant Physiol       Date:  2011-08-12       Impact factor: 8.340

7.  L-galactono-1,4-lactone dehydrogenase is required for the accumulation of plant respiratory complex I.

Authors:  Bernard Pineau; Ouardia Layoune; Antoine Danon; Rosine De Paepe
Journal:  J Biol Chem       Date:  2008-09-17       Impact factor: 5.157

8.  A structural investigation of complex I and I+III2 supercomplex from Zea mays at 11-13 A resolution: assignment of the carbonic anhydrase domain and evidence for structural heterogeneity within complex I.

Authors:  Katrin Peters; Natalya V Dudkina; Lothar Jänsch; Hans-Peter Braun; Egbert J Boekema
Journal:  Biochim Biophys Acta       Date:  2007-11-04

9.  Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1991-12       Impact factor: 3.365

10.  3D Gel Map of Arabidopsis Complex I.

Authors:  Katrin Peters; Katharina Belt; Hans-Peter Braun
Journal:  Front Plant Sci       Date:  2013-06-04       Impact factor: 5.753
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.