Literature DB >> 34541256

In vitro Assays for Measuring Endothelial Permeability byTranswells and Electrical Impedance Systems.

Hong-Ru Chen1, Trai-Ming Yeh1,2.   

Abstract

Vascular leakage is an important feature in several diseases, such as septic shock, viral hemorrhagic fever, cancer metastasis and ischemia-reperfusion injuries. Thus establishing assays for measuring endothelial permeability will provide insight into the establishment or progression of such diseases. Here, we provide transwell permeability assay and electrical impedance sensing assay for studying endothelial permeability in vitro. With these methods, the effect of a molecule on endothelial permeability could be defined.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Endothelial permeability; Transwell; Vascular leakage

Year:  2017        PMID： 34541256      PMCID： PMC8410333          DOI： 10.21769/BioProtoc.2273

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

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