Literature DB >> 27625205

Reuse of E-plate cell sensor arrays in the xCELLigence Real-Time Cell Analyzer.

Justyna Stefanowicz-Hajduk1, Anna Adamska1, Rafal Bartoszewski1, J Renata Ochocka1.   

Abstract

The xCELLigence Real-Time Cell Analyzer (RTCA) is a non-invasive, impedence-based biosensor system that can measure cell viability, migration, growth, spreading, and proliferation. Changes in cell morphology and behavior are continuously monitored in real time using microelectronics located in the wells of RTCA E-plates. According to the manufacturer's recommendation, E-plates are single-use and disposable. Here, we show that E-plates can be regenerated and reused several times without significantly effecting experimental results.

Keywords:  RTCA system; cell index; impedance; regeneration; sensor-electrodes; trypsinization

Mesh：

Year:  2016        PMID： 27625205     DOI： 10.2144/000114450

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  12 in total

1.  l-Homocysteine-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinaemia.

Authors:  Yi-Ping Leng; Ye-Shuo Ma; Xiao-Gang Li; Rui-Fang Chen; Ping-Yu Zeng; Xiao-Hui Li; Cheng-Feng Qiu; Ya-Pei Li; Zhen Zhang; Alex F Chen
Journal:  Br J Pharmacol       Date:  2017-08-11       Impact factor: 8.739

2.  Modulation of chondrocyte motility by tetrahedral DNA nanostructures.

Authors:  Sirong Shi; Shiyu Lin; Xiaoru Shao; Qianshun Li; Zhang Tao; Yunfeng Lin
Journal:  Cell Prolif       Date:  2017-08-09       Impact factor: 6.831

3.  miR-34c-5p modulates X-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response.

Authors:  Sylwia Bartoszewska; Aleksandra Cabaj; Michał Dąbrowski; James F Collawn; Rafal Bartoszewski
Journal:  FASEB J       Date:  2019-07-17       Impact factor: 5.191

4.  In vitro Assays for Measuring Endothelial Permeability byTranswells and Electrical Impedance Systems.

Authors:  Hong-Ru Chen; Trai-Ming Yeh
Journal:  Bio Protoc       Date:  2017-05-05

Review 5.  An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters.

Authors:  Vojtech Dvorak; Tabea Wiedmer; Alvaro Ingles-Prieto; Patrick Altermatt; Helena Batoulis; Felix Bärenz; Eckhard Bender; Daniela Digles; Franz Dürrenberger; Laura H Heitman; Adriaan P IJzerman; Douglas B Kell; Stefanie Kickinger; Daniel Körzö; Philipp Leippe; Thomas Licher; Vania Manolova; Riccardo Rizzetto; Francesca Sassone; Lia Scarabottolo; Avner Schlessinger; Vanessa Schneider; Hubert J Sijben; Anna-Lena Steck; Hanna Sundström; Sara Tremolada; Maria Wilhelm; Marina Wright Muelas; Diana Zindel; Claire M Steppan; Giulio Superti-Furga
Journal:  Front Pharmacol       Date:  2021-08-10       Impact factor: 5.988

6.  Galectin-7 is elevated in endometrioid (type I) endometrial cancer and promotes cell migration.

Authors:  Ellen Menkhorst; Meaghan Griffiths; Michelle Van Sinderen; Kate Rainczuk; Keith Niven; Evdokia Dimitriadis
Journal:  Oncol Lett       Date:  2018-07-23       Impact factor: 2.967

7.  Use of real-time cellular analysis and Plackett-Burman design to develop the serum-free media for PC-3 prostate cancer cells.

Authors:  Ai Zhao; Fahai Chen; Chunhong Ning; Haiming Wu; Huanfang Song; Yanqing Wu; Rong Chen; Kaihua Zhou; Xiaoling Xu; Yinxiang Lu; Jimin Gao
Journal:  PLoS One       Date:  2017-09-25       Impact factor: 3.240

8.  Circular RNA Signature Predicts Gemcitabine Resistance of Pancreatic Ductal Adenocarcinoma.

Authors:  Feng Shao; Mei Huang; Futao Meng; Qiang Huang
Journal:  Front Pharmacol       Date:  2018-06-05       Impact factor: 5.810

Review 9.  The Hitchhiker's Guide to Human Therapeutic Nanoparticle Development.

Authors:  Thelvia I Ramos; Carlos A Villacis-Aguirre; Katherine V López-Aguilar; Leandro Santiago Padilla; Claudia Altamirano; Jorge R Toledo; Nelson Santiago Vispo
Journal:  Pharmaceutics       Date:  2022-01-21       Impact factor: 6.321

Review 10.  Real-time cell analysis system in cytotoxicity applications: Usefulness and comparison with tetrazolium salt assays.

Authors:  Justyna Stefanowicz-Hajduk; J Renata Ochocka
Journal:  Toxicol Rep       Date:  2020-02-07
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