Literature DB >> 34541200

Lipidomic Analysis of Caenorhabditis elegans Embryos.

Hung-Chi Yang1,2, Cheng-Yu Hung1, Yi-Yun Pan3, Szecheng J Lo4, Daniel Tsun-Yee Chiu1,3,5,6.   

Abstract

Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is simple and homogeneous. Here, we demonstrate detailed procedures of C. elegans culture, embryo isolation, lipid extraction and metabolomic data analysis.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  C. elegans; Embryo; LC-MS; Lipid; Untargeted metabolomic

Year:  2017        PMID： 34541200      PMCID： PMC8413640          DOI： 10.21769/BioProtoc.2554

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  10 in total

Review 1.  Understanding Metabolic Regulation at a Systems Level: Metabolite Sensing, Mathematical Predictions, and Model Organisms.

Authors:  Emma Watson; L Safak Yilmaz; Albertha J M Walhout
Journal:  Annu Rev Genet       Date:  2015       Impact factor: 16.830

2.  Comparative Metabolomic Profiling Reveals That Dysregulated Glycolysis Stemming from Lack of Salvage NAD+ Biosynthesis Impairs Reproductive Development in Caenorhabditis elegans.

Authors:  Wenqing Wang; Melanie R McReynolds; Jimmy F Goncalves; Muya Shu; Ineke Dhondt; Bart P Braeckman; Stephanie E Lange; Kelvin Kho; Ariana C Detwiler; Marisa J Pacella; Wendy Hanna-Rose
Journal:  J Biol Chem       Date:  2015-09-08       Impact factor: 5.157

3.  Meta-analysis of global metabolomic data identifies metabolites associated with life-span extension.

Authors:  Gary J Patti; Ralf Tautenhahn; Darcy Johannsen; Ewa Kalisiak; Eric Ravussin; Jens C Brüning; Andrew Dillin; Gary Siuzdak
Journal:  Metabolomics       Date:  2014-08-01       Impact factor: 4.290

4.  Comparison of proteomic and metabolomic profiles of mutants of the mitochondrial respiratory chain in Caenorhabditis elegans.

Authors:  P G Morgan; R Higdon; N Kolker; A T Bauman; O Ilkayeva; C B Newgard; E Kolker; L M Steele; M M Sedensky
Journal:  Mitochondrion       Date:  2014-12-19       Impact factor: 4.160

5.  A comparative metabolomic study of NHR-49 in Caenorhabditis elegans and PPAR-alpha in the mouse.

Authors:  Helen J Atherton; Oliver A H Jones; Shahid Malik; Eric A Miska; Julian L Griffin
Journal:  FEBS Lett       Date:  2008-04-22       Impact factor: 4.124

6.  The metabolomic responses of Caenorhabditis elegans to cadmium are largely independent of metallothionein status, but dominated by changes in cystathionine and phytochelatins.

Authors:  Samantha L Hughes; Jacob G Bundy; Elizabeth J Want; Peter Kille; Stephen R Stürzenbaum
Journal:  J Proteome Res       Date:  2009-07       Impact factor: 4.466

7.  A metabolomic strategy defines the regulation of lipid content and global metabolism by Δ9 desaturases in Caenorhabditis elegans.

Authors:  Cecilia Castro; Funda Sar; W Robert Shaw; Masanori Mishima; Eric A Miska; Julian L Griffin
Journal:  BMC Genomics       Date:  2012-01-20       Impact factor: 3.969

8.  Metabolomic signature associated with reproduction-regulated aging in Caenorhabditis elegans.

Authors:  Qin-Li Wan; Xiaohuo Shi; Jiangxin Liu; Ai-Jun Ding; Yuan-Zhu Pu; Zhigang Li; Gui-Sheng Wu; Huai-Rong Luo
Journal:  Aging (Albany NY)       Date:  2017-02-06       Impact factor: 5.682

9.  Impaired embryonic development in glucose-6-phosphate dehydrogenase-deficient Caenorhabditis elegans due to abnormal redox homeostasis induced activation of calcium-independent phospholipase and alteration of glycerophospholipid metabolism.

Authors:  Tzu-Ling Chen; Hung-Chi Yang; Cheng-Yu Hung; Meng-Hsin Ou; Yi-Yun Pan; Mei-Ling Cheng; Arnold Stern; Szecheng J Lo; Daniel Tsun-Yee Chiu
Journal:  Cell Death Dis       Date:  2017-01-12       Impact factor: 8.469

10.  Glucose 6-phosphate dehydrogenase deficiency enhances germ cell apoptosis and causes defective embryogenesis in Caenorhabditis elegans.

Authors:  H-C Yang; T-L Chen; Y-H Wu; K-P Cheng; Y-H Lin; M-L Cheng; H-Y Ho; S J Lo; D T-Y Chiu
Journal:  Cell Death Dis       Date:  2013-05-02       Impact factor: 8.469
  10 in total

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