Literature DB >> 34541194

Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2',7'-Dichlorodihydrofluorescein Diacetate (DCFH-DA).

Jainendra Pathak1, Ananya Chatterjee1, Shailendra P Singh1, Rajeshwar P Sinha1.   

Abstract

Reactive oxygen species (ROS) are cell signaling molecules synthesized inside the cells as a response to routine metabolic processes. In stress conditions such as ultraviolet radiation (UVR), ROS concentration increases several folds in the cells that become toxic for the cell survival. Here we present the method for in vivo detection of ROS by using an oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in cyanobacteria. This method provides reliable, simple, rapid and cost effective means for detection of ROS in cyanobacteria.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  2’; 7’-Dichlorodihydrofluorescein diacetate; Cyanobacteria; Oxidative damage; Reactive oxygen species; Ultraviolet radiation

Year:  2017        PMID： 34541194      PMCID： PMC8413551          DOI： 10.21769/BioProtoc.2545

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


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