Literature DB >> 34541105

Targeted Mutagenesis Using RNA-guided Endonucleases in Mosses.

Toshihisa Nomura1, Hitoshi Sakakibara1,2.   

Abstract

RNA-guided endonucleases (RGENs) have been used for genome editing in various organisms. Here, we demonstrate a simple method for performing targeted mutagenesis and genotyping in a model moss species, Physcomitrella patens, using RGENs. We also performed targeted mutagenesis in a non-model moss, Scopelophilla cataractae, using a similar method ( Nomura et al., 2016 ), indicating that this experimental system could be applied to a wide range of mosses species.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  CRISPR/Cas system; Genome editing; Mosses; RNA-guided endonucleases; Targeted mutagenesis

Year:  2017        PMID： 34541105      PMCID： PMC8410312          DOI： 10.21769/BioProtoc.2359

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  9 in total

1.  CasOT: a genome-wide Cas9/gRNA off-target searching tool.

Authors:  An Xiao; Zhenchao Cheng; Lei Kong; Zuoyan Zhu; Shuo Lin; Ge Gao; Bo Zhang
Journal:  Bioinformatics       Date:  2014-01-02       Impact factor: 6.937

2.  Class 1 KNOX genes are not involved in shoot development in the moss Physcomitrella patens but do function in sporophyte development.

Authors:  Keiko Sakakibara; Tomoaki Nishiyama; Hironori Deguchi; Mitsuyasu Hasebe
Journal:  Evol Dev       Date:  2008 Sep-Oct       Impact factor: 1.930

3.  Efficient and Heritable Targeted Mutagenesis in Mosses Using the CRISPR/Cas9 System.

Authors:  Toshihisa Nomura; Tetsuya Sakurai; Yuriko Osakabe; Keishi Osakabe; Hitoshi Sakakibara
Journal:  Plant Cell Physiol       Date:  2016-10-22       Impact factor: 4.927

4.  Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

Authors:  John G Doench; Ella Hartenian; Daniel B Graham; Zuzana Tothova; Mudra Hegde; Ian Smith; Meagan Sullender; Benjamin L Ebert; Ramnik J Xavier; David E Root
Journal:  Nat Biotechnol       Date:  2014-09-03       Impact factor: 54.908

5.  Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis.

Authors:  T Nishiyama; Y Hiwatashi; I Sakakibara; M Kato; M Hasebe
Journal:  DNA Res       Date:  2000-02-28       Impact factor: 4.458

6.  A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Authors:  Martin Jinek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2012-06-28       Impact factor: 47.728

7.  Primer3--new capabilities and interfaces.

Authors:  Andreas Untergasser; Ioana Cutcutache; Triinu Koressaar; Jian Ye; Brant C Faircloth; Maido Remm; Steven G Rozen
Journal:  Nucleic Acids Res       Date:  2012-06-22       Impact factor: 16.971

8.  Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

Authors:  Yanfang Fu; Jeffry D Sander; Deepak Reyon; Vincent M Cascio; J Keith Joung
Journal:  Nat Biotechnol       Date:  2014-01-26       Impact factor: 54.908

9.  Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants.

Authors:  Yuriko Osakabe; Takahito Watanabe; Shigeo S Sugano; Risa Ueta; Ryosuke Ishihara; Kazuo Shinozaki; Keishi Osakabe
Journal:  Sci Rep       Date:  2016-05-26       Impact factor: 4.379
  9 in total

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