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Literature DB >> 34541080

Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen.

Puneet Paul^1,2, Palak Chaturvedi³, Anida Mesihovic¹, Arindam Ghatak³, Wolfram Weckwerth^3,4, Enrico Schleiff^1,5,6.

Abstract

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.

Entities: Chemical

Keywords: Membrane proteome; Pollen; Proteomics; Tomato

Year: 2017 PMID： 34541080 PMCID： PMC8410314 DOI： 10.21769/BioProtoc.2315

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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References

12 in total

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Review 2. Importance of organellar proteins, protein translocation and vesicle transport routes for pollen development and function.

Authors: Puneet Paul; Sascha Röth; Enrico Schleiff
Journal: Plant Reprod Date: 2016-02-13 Impact factor: 3.767

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Authors: Luis Valledor; Wolfram Weckwerth
Journal: Methods Mol Biol Date: 2014

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Authors: Heidi Pertl; Waltraud X Schulze; Gerhard Obermeyer
Journal: J Proteome Res Date: 2009-11 Impact factor: 4.466

5. Chaperone network composition in Solanum lycopersicum explored by transcriptome profiling and microarray meta-analysis.

Authors: Sotirios Fragkostefanakis; Stefan Simm; Puneet Paul; Daniela Bublak; Klaus-Dieter Scharf; Enrico Schleiff
Journal: Plant Cell Environ Date: 2014-10-01 Impact factor: 7.228

6. Cell-specific analysis of the tomato pollen proteome from pollen mother cell to mature pollen provides evidence for developmental priming.

Authors: Palak Chaturvedi; Till Ischebeck; Volker Egelhofer; Irene Lichtscheidl; Wolfram Weckwerth
Journal: J Proteome Res Date: 2013-07-11 Impact factor: 4.466

Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen.

1. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.

Review 2. Importance of organellar proteins, protein translocation and vesicle transport routes for pollen development and function.

3. An improved detergent-compatible gel-fractionation LC-LTQ-Orbitrap-MS workflow for plant and microbial proteomics.

4. The pollen organelle membrane proteome reveals highly spatial-temporal dynamics during germination and tube growth of lily pollen.

5. Chaperone network composition in Solanum lycopersicum explored by transcriptome profiling and microarray meta-analysis.

6. Cell-specific analysis of the tomato pollen proteome from pollen mother cell to mature pollen provides evidence for developmental priming.

7. ARAMEMNON, a novel database for Arabidopsis integral membrane proteins.

Review 8. Pollen proteomics: from stress physiology to developmental priming.

9. The membrane proteome of male gametophyte in Solanum lycopersicum.

10. The protein translocation systems in plants - composition and variability on the example of Solanum lycopersicum.