| Literature DB >> 34520018 |
Irene Chiesa1,2,3,4, Roberto Di Gesù3,4,5, Kalon J Overholt4,6,7,8, Riccardo Gottardi9,10,11.
Abstract
Interface tissues are functionally graded tissues characterized by a complex layered structure, which therefore present a great challenge to be reproduced and cultured in vitro. Here, we describe the design and operation of a 3D printed dual-chamber bioreactor as a culturing system for biphasic native or engineered osteochondral tissues. The bioreactor is designed to potentially accommodate a variety of interface tissues and enables the precise study of tissue crosstalk by creating two separate microenvironments while maintaining the tissue compartments in direct contact.Entities:
Keywords: Bioreactor; Biphasic constructs; Crosstalk; Interface tissue engineering; Microphysiological system; Osteochondral junction; Paracrine signaling
Mesh：
Year: 2022 PMID： 34520018 DOI： 10.1007/978-1-0716-1693-2_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745